Obiettivo My project focuses on the characterization of the mechanism of action of ESCRT-III complex. ESCRTs are evolutionary conserved proteins which mediate membrane remodeling and scission in a variety of important cellular processes, and are implicated in the pathogenesis of many diseases, including virus release and nuclear membrane repair during cancer cell migration. Despite intense research, many outstanding questions still remain unanswered, and all model proposed remain highly speculative. Based on our preliminary data, we propose a novel, unifying model for ESCRT-III function. The aim of the project is to provide solid data to support this model by an in vitro reconstitution approach, which would constitute a major contribution in the field. The concrete aims of my project are:- Characterization of the minimal ESCRT-III fission machinery - Characterization of the molecular mechanism leading to membrane fission by ESCRT-III complex- Characterization of the membrane requirements to obtain fission by ESCRT-III, in particular membrane curvature Campo scientifico natural sciencesbiological sciencesmicrobiologyvirologynatural sciencesmathematicspure mathematicstopologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural sciencesmathematicspure mathematicsgeometry Parole chiave ESCRT-III membrane scission cytokinesis MVB HIV budding membrane repair CHMP negative membrane curvature Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2016 - Individual Fellowships Invito a presentare proposte H2020-MSCA-IF-2016 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinatore INSTITUT CURIE Contribution nette de l'UE € 185 076,00 Indirizzo RUE D ULM 26 75231 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 185 076,00
