Constructing and powering nanoscale DNA origami motors

Projektinformationen

DNA ORIGAMI MOTORS

ID Finanzhilfevereinbarung: 724261

DOI 10.3030/724261

Abgeschlossenes Projekt

EK-Unterschriftsdatum 4 April 2017

Startdatum 1 Mai 2017

Enddatum 30 April 2022

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten € 2 000 000,00

EU-Beitrag € 2 000 000,00

Koordiniert durch

TECHNISCHE UNIVERSITAET MUENCHEN
Germany

Ziel

Our goal is to advance the field of DNA nanotechnology by achieving directed transport on the nanoscale using robustly functioning synthetic motor units. To do so, we propose to construct spatially periodic, diffusive mechanisms that have broken inversion symmetry and to subject these mechanisms to conditions away from thermal equilibrium. We will build on recent progress in creating complex DNA-based structures and construct various nanoscale rotary and translational Brownian ratchet mechanisms that have well- defined degrees of freedom for motion within periodic and asymmetric energy landscapes. The mechanisms will be self-assembled from DNA origami components. We will use cryo-Transmission Electron Microscopy (TEM) to evaluate and iteratively refine our structures. Conventional video-rate fluorescence microscopy, in addition to super-resolution microscopy, will be employed to study in solution and in real time the diffusive motion of the mechanisms on the single particle level. We will introduce various deterministic or stochastic thermal, mechanical, or chemical perturbations to drive the systems away from thermal equilibrium. We will use laser heating and cooling to experimentally test thermal and flashing ratcheting mechanisms; we will employ dissipative asymmetric fluxes arising in active matter as realized in high-density ATP-hydrolysing motility assays; and we will couple out-of-equilibrium chemical reactions to the motion of our mechanisms. The ultimate goal of our work is to take insights from these experiments and create robustly functioning nanoscale motor units that can drive directed motion against external load and perform at levels comparable to those of natural macromolecular motor proteins. Achieving this goal will create unprecedented technological opportunities, for example, to drive chemical synthesis, actively propel nanoscale drug- delivery vehicles, pump and separate molecules across barriers or package molecules into cargo components.

Wissenschaftliches Gebiet

• natural sciences › biological sciences › genetics › DNA
• natural sciences › physical sciences › optics › microscopy › super resolution microscopy
• natural sciences › physical sciences › optics › microscopy › electron microscopy
• natural sciences › chemical sciences
• natural sciences › physical sciences › optics › laser physics

Programm/Programme

• H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme

Thema/Themen

• ERC-2016-COG - ERC Consolidator Grant

Aufforderung zur Vorschlagseinreichung

ERC-2016-COG

Finanzierungsplan

ERC-COG - Consolidator Grant

Gastgebende Einrichtung

TECHNISCHE UNIVERSITAET MUENCHEN

• Netto-EU-Beitrag: € 2 000 000,00
• Adresse: Arcisstrasse 21 ; 80333 Muenchen ; Deutschland
• Region: Bayern › Oberbayern › München, Kreisfreie Stadt
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 2 000 000,00

Begünstigte (1)

TECHNISCHE UNIVERSITAET MUENCHEN

Deutschland

• Netto-EU-Beitrag: € 2 000 000,00
• Adresse: Arcisstrasse 21 ; 80333 Muenchen
• Region: Bayern › Oberbayern › München, Kreisfreie Stadt
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 2 000 000,00