Objectif Flowering plants are the most successful plant group on Earth and provide the basis for most human and animal food. The development of flowers is dependent on a gene regulatory network orchestrated by LEAFY (LFY), the master transcription factor. In Arabidopsis as in other flowering plants, LFY activity is dependent upon its interaction with UFO, a member of the Skp1–Cullin–F-box E3 ubiquitin ligase complex. The goal of this project is to understand why LFY requires interacting with UFO to build flowers. For this, we will apply state-of-the-art technologies including mass-spectrometry, crystallography, biochemistry and plant developmental genetics to identify and characterize the function of Post-Translational Modifications (PTM) on LFY or LFY interacting proteins, with special emphasis on ubiquitination. After identifying key residues involved in the LFY-UFO interaction or targeted by PTM, their functional importance will be assayed in planta. This project should facilitate better understanding of the mechanism of LFY activation. LEAFY and the development of flower will be used here as a model to understand the “activation by degradation” mechanism, still elusive in most organisms and poorly studied in plants. Moreover, understanding the principles governing LFY activation should provide means to modify at will the architecture of plant inflorescences. This project builds on the current background of the applicant in the field of Mass-Spectrometry combined with the outstanding expertise at Grenoble and high-throughput facilities in Structural biology and plant developmental genetics. Notably, the applicant will work within one of the top French Research Centers and in a multidisciplinary environment that will allow the fellow to acquire complementary ‘new knowledge and skills’ in structural biology and developmental genetics. This will re-enforce the fellow’s background and professional maturity for employment opportunities in both academia and industry sectors. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesearth and related environmental sciencesgeologymineralogycrystallographymedical and health scienceshealth sciencesnutritionnatural scienceschemical sciencesorganic chemistryaminesnatural sciencesbiological sciencesmolecular biologystructural biology Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2016 - Individual Fellowships Appel à propositions H2020-MSCA-IF-2016 Voir d’autres projets de cet appel Régime de financement MSCA-IF-EF-ST - Standard EF Coordinateur COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES Contribution nette de l'UE € 185 076,00 Adresse RUE LEBLANC 25 75015 PARIS 15 France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 185 076,00
