Objetivo Recent breakthroughs in the field of genome editing provide a genuine opportunity to establish innovativeapproaches to repair DNA mutations to replace, engineer or regenerate malfunctioning cells in vitro or invivo. However, most of the recently developed technologies introduce double-strand DNA breaks at a targetlocus as the first step to gene correction. These breaks are subsequently repaired by one of the cell intrinsicDNA repair pathways, typically inducing an abundance of insertions and deletions (indels). Ideally, for manyapplications genome editing should, however, be efficient and specific, without the introduction of indels.Site-specific recombinases (SSRs) allow precise genome editing without triggering endogenous DNA repairpathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processedDNA in vivo. However, customizing the DNA binding specificity of SSRs is not straightforward. With thisproject, we propose to solve this shortcoming. We have already demonstrated that by applying substrate-linkeddirected evolution, SSRs can be generated that specifically recognize therapeutic targets. Theobjective of this project is the development of a universal genome editing platform that allows flexible,efficient and safe gene corrections in cells of any origin without triggering cell intrinsic DNA repair.GenSurge aims to: i) sequence an unprecedented, comprehensive compendium of evolved SSRs tounderstand the directed molecular evolution process at nucleotide resolution; ii) integrate the knowledgeobtained in i) to develop a unique SSR-based approach to correct genomic inversions; iii) develop auniversal SSR-based strategy that allows flawless, precise and safe genome editing to correct any gene defectin human, animal or plant cells. The successful implementation of this project will deliver a comprehensive,safe and efficient platform from which genome surgery-based cure strategies can be initiated. Ámbito científico natural sciencesbiological sciencesgeneticsgenomes Palabras clave genetic engineering genome editing side-specific recombination Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-2016-ADG - ERC Advanced Grant Convocatoria de propuestas ERC-2016-ADG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-ADG - Advanced Grant Institución de acogida TECHNISCHE UNIVERSITAET DRESDEN Aportación neta de la UEn € 2 380 425,00 Dirección HELMHOLTZSTRASSE 10 01069 Dresden Alemania Ver en el mapa Región Sachsen Dresden Dresden, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 380 425,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo TECHNISCHE UNIVERSITAET DRESDEN Alemania Aportación neta de la UEn € 2 380 425,00 Dirección HELMHOLTZSTRASSE 10 01069 Dresden Ver en el mapa Región Sachsen Dresden Dresden, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 380 425,00
