Objetivo The aim of this grant is to understand how cellular, tissue-scale and organismal left-right asymmetry arisesfrom the chirality of molecular constituents. In many instances the actomyosin cortex, a thin andmechanically active layer of dynamically cross-linked filaments and molecular motors at the surface of cells,drives the emergence of chiral morphogenetic events. In the nematode Caenorhabditis elegans, mesoscalechiral active torques generated by this active layer establish the embryo’s left-right body axis. Here we wantto understand how mesoscale actomyosin active torques are generated at the molecular level, and how activetorque generation in the actomyosin surface drives chiral morphogenesis of cells, tissues and organisms.Cells and tissues represent a new class of active chiral materials where both the force and the torque balanceneed to be considered, and we will perform a systematic and cross-scale characterization of active chiralbiological matter. We will pursue an interdisciplinary approach at the interface of physics and biology. At themolecular-scale, we will use optical tweezers to measure active torques generated by single molecules of themolecular myosin and the actin polymerizing protein formin. At the cell-scale, we will reconstitute chiralactomyosin flows in vitro and characterize chiral dynamics of single molecules in vivo. At the tissue-scale,we will investigate chiral cell movements in a multicellular environment and unravel the physical basis ofchiral tissue flow in vertebrates. Theory is essential at all stages, and we will build a molecular-scale modelof actomyosin torque generation that will be coarse-grained to a generalized hydrodynamic description ofactive chiral matter. This interdisciplinary and cross-scale approach will provide fundamentally new insightsinto active chiral materials and the mechanisms by which left-right asymmetries arise in development. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesclinical medicineembryology Palabras clave Actomyosin Torque generation Chiral patterning Left Right asymmetry Morphogenesis Biophysics Modelling Theory Cell biology C.elegans Chicken Quail Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-2016-ADG - ERC Advanced Grant Convocatoria de propuestas ERC-2016-ADG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-ADG - Advanced Grant Coordinador TECHNISCHE UNIVERSITAET DRESDEN Aportación neta de la UEn € 2 500 000,00 Dirección Helmholtzstrasse 10 01069 Dresden Alemania Ver en el mapa Región Sachsen Dresden Dresden, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 500 000,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo TECHNISCHE UNIVERSITAET DRESDEN Alemania Aportación neta de la UEn € 2 500 000,00 Dirección Helmholtzstrasse 10 01069 Dresden Ver en el mapa Región Sachsen Dresden Dresden, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 2 500 000,00