Objetivo Temperature is crucial to plants’ growth and development. The cold and heat stresses may drastically inhibit plant growth and cause yield losses in crops. Understanding the temperature responding mechanism is crucial to adapt the crops to withstand extreme temperature changes caused by global warming. Among other temperature regulation mechanism, RNA thermometers (RNATs) can instantaneously respond to temperature shift and directly control translational efficiency therefore protein abundance. To date, no RNAT has been identified in plant mainly due to the fact that instead of sequence level conservation, RNATs only have structure level conservation; also because in-vivo RNA structure probing used to be technically challenging. The host lab has developed a powerful platform for in vivo RNA secondary structure probing at both targeted individual RNA and genome-wide scale. This proposed study aims to globally identify RNA thermometers in plants for the first time, and to quantitatively elucidate the role of RNA structure in the post-transcriptional regulation of gene expression in response to temperature. I will measure the alteration of in vivo RNA structural features in Arabidopsis thaliana under different temperature regime. Additionally, Ribosome profiling data and RNA-Seq data will be combined to study the translation efficiency under the corresponding temperature. Together with the identified significantly changed RNA structure elements, we will be able to determine the putative RNAT. Finally, I will validate the predicted RNATs through studying nature variations. I will perform in-vivo RNA structure probing and determine whether the identified single nucleotide variations (SNV) will have significant effects on temperature-regulatory elements. Besides the potential for crop improvement, our approach to globally measure RNA secondary structure and the corresponding translation efficiency will be easily applied to other organisms and various environmental stimuli. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencescomputer and information sciencesdata sciencedata miningnatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesgeneticsRNA Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2016 - Individual Fellowships Convocatoria de propuestas H2020-MSCA-IF-2016 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-ST - Standard EF Coordinador JOHN INNES CENTRE Aportación neta de la UEn € 183 454,80 Dirección NORWICH RESEARCH PARK COLNEY NR4 7UH Norwich Reino Unido Ver en el mapa Región East of England East Anglia Breckland and South Norfolk Tipo de actividad Research Organisations Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 183 454,80