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Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes

Objective

The aim of Project INDEX is to isolate and characterize nanoparticles available in bodily fluids through development and integration of novel technological breakthroughs. The technology will enable the analysis of clinically valuable nanoparticles called exosomes towards new generation diagnostics. Exosomes are known to mediate communication between cells and their effective utilization holds a great promise of revolutionizing the standard of clinical care. However, their detection and molecular profiling is technically challenging. The proposed technology will isolate exosomes that are as small as 30nm in diameter from human plasma with high purity, and provide in-depth, multi-parameter characterization of the particles through digital counting, size determination, and biological phenotyping.
Towards this goal: (1) Novel microfluidics will be developed and used for efficient magnetic enrichment; (2) Isolated particles will be detected and analyzed with a novel biological nanoparticle (BNP) sensor (3) Immune-capture and release chemistries as well as phenotyping assays will be developed; (4) Critically, complete on-chip integration of isolation, detection and analysis will be accomplished; (5) Utility of in-depth exosome characterization will be demonstrated with clinical samples for lung cancer.
Project INDEX requires successful integration of multiple sub-units and assays that each represents technological frontiers, which is extremely challenging. However, the breadth of information on exosomes that will be available with the integrated system is unmatched. Although, the clinical utility of exosomes is still developing, the uncertainty can only be clarified through automated technologies that provide latitude of information. Once completed, Project INDEX can demonstrate a new paradigm in cancer diagnostics, and also present a potential future technology for other applications involving nanoparticles.

Call for proposal

H2020-FETOPEN-2016-2017

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Sub call

H2020-FETOPEN-1-2016-2017

Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Net EU contribution
€ 758 750,00
Address
Piazzale Aldo Moro 7
00185 Roma
Italy

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Region
Centro (IT) Lazio Roma
Activity type
Research Organisations
Non-EU contribution
€ 0,00

Participants (6)

THE TRUSTEES OF BOSTON UNIVERSITY
United States
Net EU contribution
€ 708 500,00
Address
Commonwealth Avenue 881
02215 Boston Ma

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Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
INSTITUT CURIE
France
Net EU contribution
€ 417 725,00
Address
Rue D'ulm 26
75231 Paris

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Non-EU contribution
€ 0,00
Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
Net EU contribution
€ 98 437,50
Address
Rue Michel Ange 3
75794 Paris

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Non-EU contribution
€ 0,00
REGION NORDJYLLAND (NORTH DENMARK REGION)
Denmark
Net EU contribution
€ 310 000,00
Address
Niels Bohrs Vej 30
9220 Aalborg

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Region
Danmark Nordjylland Nordjylland
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
Non-EU contribution
€ 0,00
FLUIGENT SA
France
Net EU contribution
€ 473 862,50
Address
57-77 Avenue De Fontainebleau
94270 Le Kremlin-bicetre

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Ile-de-France Ile-de-France Val-de-Marne
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 1 250,00
HANSABIOMED LIFE SCIENCES OU
Estonia
Net EU contribution
€ 215 000,00
Address
Maealuse Tn 2/1, Harju Maakond, Mustamae Linnaosa
12618 Tallinn

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Region
Eesti Eesti Põhja-Eesti
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00