Skip to main content

Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes

Objective

The aim of Project INDEX is to isolate and characterize nanoparticles available in bodily fluids through development and integration of novel technological breakthroughs. The technology will enable the analysis of clinically valuable nanoparticles called exosomes towards new generation diagnostics. Exosomes are known to mediate communication between cells and their effective utilization holds a great promise of revolutionizing the standard of clinical care. However, their detection and molecular profiling is technically challenging. The proposed technology will isolate exosomes that are as small as 30nm in diameter from human plasma with high purity, and provide in-depth, multi-parameter characterization of the particles through digital counting, size determination, and biological phenotyping.
Towards this goal: (1) Novel microfluidics will be developed and used for efficient magnetic enrichment; (2) Isolated particles will be detected and analyzed with a novel biological nanoparticle (BNP) sensor (3) Immune-capture and release chemistries as well as phenotyping assays will be developed; (4) Critically, complete on-chip integration of isolation, detection and analysis will be accomplished; (5) Utility of in-depth exosome characterization will be demonstrated with clinical samples for lung cancer.
Project INDEX requires successful integration of multiple sub-units and assays that each represents technological frontiers, which is extremely challenging. However, the breadth of information on exosomes that will be available with the integrated system is unmatched. Although, the clinical utility of exosomes is still developing, the uncertainty can only be clarified through automated technologies that provide latitude of information. Once completed, Project INDEX can demonstrate a new paradigm in cancer diagnostics, and also present a potential future technology for other applications involving nanoparticles.

Field of science

  • /medical and health sciences/clinical medicine/oncology/cancer
  • /natural sciences/physical sciences/theoretical physics/particles

Call for proposal

H2020-FETOPEN-1-2016-2017
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Address
Piazzale Aldo Moro 7
00185 Roma
Italy
Activity type
Research Organisations
EU contribution
€ 758 750

Participants (5)

THE TRUSTEES OF BOSTON UNIVERSITY
United States
EU contribution
€ 708 500
Address
Commonwealth Avenue 881
02215 Boston Ma
Activity type
Higher or Secondary Education Establishments
INSTITUT CURIE
France
EU contribution
€ 516 162,50
Address
Rue D'ulm 26
75231 Paris
Activity type
Research Organisations
REGION NORDJYLLAND (NORTH DENMARK REGION)
Denmark
EU contribution
€ 310 000
Address
Niels Bohrs Vej 30
9220 Aalborg
Activity type
Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments)
FLUIGENT SA
France
EU contribution
€ 473 862,50
Address
57-77 Avenue De Fontainebleau
94270 Le Kremlin-bicetre
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
HANSABIOMED LIFE SCIENCES OU
Estonia
EU contribution
€ 215 000
Address
Maealuse Tn 2/1, Harju Maakond, Mustamae Linnaosa
12618 Tallinn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)