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Light-controlled synthetic enzyme cascades

Light-controlled synthetic enzyme cascades

Objective

There is an urgent need for the development of greener syntheses procedures if mankind wants to maintain an environment worth living in but is at the same time unwilling to accept a reduction in material comfort. The establishment of more biocatalytic steps in chemical syntheses is one possible solution, as enzymes and whole cells offer sustainable advantages, such as biodegradability, intoxicity, high selectivity, and many more. As a myriad of enzymatic reactions exist for almost any product, their potential is immense. Great scientific achievements and new techniques recently developed have enabled the design of economically and ecologically feasible multi-step enzyme cascades. However, with these new opportunities, also new challenges arise. The more enzyme steps are combined in one pot, the higher the risk of undesired cross-reactivity is. There is thus an urgent need for a tight control of each biocatalytic step in a cascade in order to obtain the desired product in a high purity and to make use of all advantages that enzyme cascades intrinsically offer. With LightCas, I aim to break new grounds in the area of multi-step (bio)catalysis by enabling an orthogonal, selective and thus flexible on/off tuning of enzymes in a cascade. By entrapping enzymes into light-switchable microgels, using photo-switchable active site lids and light-induced enzyme deactivation, three methods providing the opportunity to control enzyme activity in vitro and in vivo on demand will be (further) developed. The ultimate goal is to set up a one-pot multi-step light-controlled enzyme reactor yielding the desired product in high selectivity and concentration in a technically self-regulated manner.
Beyond the ground-breaking direct impact in the field of enzyme catalysis, huge gains in knowledge are expected from LightCas with respect to the application of intelligent stimuli-responsive materials as well as new, advanced methods for applications in the clinical and research environment.
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Host institution

FORSCHUNGSZENTRUM JULICH GMBH

Address

Wilhelm Johnen Strasse
52428 Julich

Germany

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 498 125

Beneficiaries (1)

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FORSCHUNGSZENTRUM JULICH GMBH

Germany

EU Contribution

€ 1 498 125

Project information

Grant agreement ID: 757320

Status

Ongoing project

  • Start date

    1 January 2018

  • End date

    31 December 2022

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 498 125

  • EU contribution

    € 1 498 125

Hosted by:

FORSCHUNGSZENTRUM JULICH GMBH

Germany