Engineering synthetic pentatricopeptide repeat proteins for the site-specific genetic manipulation of plant organelles

Projektinformationen

PPRdesign

ID Finanzhilfevereinbarung: 794377

DOI 10.3030/794377

Abgeschlossenes Projekt

EK-Unterschriftsdatum 19 März 2018

Startdatum 1 Juni 2018

Enddatum 31 Mai 2020

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 185 076,00

EU-Beitrag € 185 076,00

Koordiniert durch

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France

Ziel

Mitochondria and chloroplasts are essential organelles that carry out the fundamental cellular process of respiration and photosynthesis, respectively. Organelles possess their own genomes that are physically separated from the nuclear genes, and mutations in their genome lead to serious consequences on organismal homeostasis and viability e.g. flower sterility in plants and mitochondrial myopathies in humans. However, contrary to the nucleus, methods for the genetic engineering of organellar genomes in multicellular organisms are either lacking or too laborious to be widely applied. Thus, there is a need for the development of such tools. To address this issue, I will exploit nature’s design and engineer the largest protein family of organelle gene regulators found in eukaryotes, the pentatricopeptide repeat (PPR) family. PPR proteins are nuclear encoded RNA-binding proteins that function in the posttranscriptional regulation of organellar gene expression. The PPR RNA-binding domain is composed of multiple repeats of 35 amino acid long stretches. Each repeat recognizes a single base in the target RNA sequence whose identity is defined by a code involving 2 amino acids in each repeat and we can now design artificial PPR repeat proteins with programmable RNA binding specificity. The PPRdesign project will establish new methods for the site-specific control of organellar genes in the Arabidopsis model plant by targeting customized synthetic PPR repeats to specific RNA sites in chloroplasts and mitochondria.

Wissenschaftliches Gebiet

• medical and health sciences › medical biotechnology › genetic engineering
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › genetics › RNA
• natural sciences › chemical sciences › organic chemistry › amines
• natural sciences › biological sciences › genetics › genomes

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2017 - Individual Fellowships

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2017

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

• Netto-EU-Beitrag: € 185 076,00
• Adresse: RUE MICHEL ANGE 3 ; 75794 Paris ; Frankreich
• Region: Ile-de-France › Ile-de-France › Paris
• Aktivitätstyp: Research Organisations
• Gesamtkosten: € 185 076,00