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Chromatin organization PROfiling with high-throughput super-resolution microscopy on a CHIP

Objective

Cancer is both a genetic and an epigenetic disease whose outcome is influenced by tumor microenvironment, which represents the major driving force of tumorigenesis causing the functional heterogeneity observed in most cancer types. Defining the 3D-organization of cancer-associated chromatin domains would represent a new frontier to decipher tumor heterogeneity. None of the currently available technologies permit to rapidly analyze thousands of cells and profile their chromatin organization at single cell level, as needed for medical diagnosis and therapeutic guidance.
The goal of the project is to build a high-throughput super-resolution microscope in a microfluidic chip smaller than a coin. With this device we will provide high resolution imaging of hundreds of cells at the diffraction limit and beyond, with minimal photo-toxicity.
Femtosecond laser micromachining allows fabricating with accurate precision optofluidic components as waveguides, microchannels and lenses in a glass substrate. We will integrate them in a single chip, to achieve the required illumination path for advanced fluorescence excitation and sample movement: in the same chip biological samples will be scanned along fluidic channels in a fully automatic fashion.
High-throughput data on chromatin distribution in hundreds of samples will be generated, allowing to decipher the pathogenic function of tumor heterogeneities in tumor progression. These data will be used as benchmarks for predicting differential responsiveness and/or resistance of cancer cells to targeted therapies opening brand new possibilities for medical diagnosis and therapeutic guidance.
The consortium is formed by young scientists from Universities in the field of photonics, computer sciences and epigenetics, and a leading company in microfluidics.

Call for proposal

H2020-FETOPEN-1-2016-2017
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Funding Scheme

RIA - Research and Innovation action

Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Address
Piazzale Aldo Moro 7
00185 Roma
Italy
Activity type
Research Organisations
EU contribution
€ 601 250

Participants (5)

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
United Kingdom
EU contribution
€ 511 880
Address
South Kensington Campus Exhibition Road
SW7 2AZ London
Activity type
Higher or Secondary Education Establishments
UNIVERSITA DEGLI STUDI DI TRENTO
Italy
EU contribution
€ 492 873,75
Address
Via Calepina 14
38122 Trento
Activity type
Higher or Secondary Education Establishments
INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE LYON
France
EU contribution
€ 360 250
Address
20 Avenue Albert Einstein
69621 Villeurbanne Cedex
Activity type
Higher or Secondary Education Establishments
ELVESYS
France
EU contribution
€ 499 271,25
Address
172 Rue De Charonne B2 2 Eme Etage
75011 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
UNIVERSITE D'ANGERS
France
EU contribution
€ 31 000
Address
Rue De Rennes 40
49035 Angers Cedex 01
Activity type
Higher or Secondary Education Establishments