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Chromatin organization PROfiling with high-throughput super-resolution microscopy on a CHIP

Objective

Cancer is both a genetic and an epigenetic disease whose outcome is influenced by tumor microenvironment, which represents the major driving force of tumorigenesis causing the functional heterogeneity observed in most cancer types. Defining the 3D-organization of cancer-associated chromatin domains would represent a new frontier to decipher tumor heterogeneity. None of the currently available technologies permit to rapidly analyze thousands of cells and profile their chromatin organization at single cell level, as needed for medical diagnosis and therapeutic guidance.
The goal of the project is to build a high-throughput super-resolution microscope in a microfluidic chip smaller than a coin. With this device we will provide high resolution imaging of hundreds of cells at the diffraction limit and beyond, with minimal photo-toxicity.
Femtosecond laser micromachining allows fabricating with accurate precision optofluidic components as waveguides, microchannels and lenses in a glass substrate. We will integrate them in a single chip, to achieve the required illumination path for advanced fluorescence excitation and sample movement: in the same chip biological samples will be scanned along fluidic channels in a fully automatic fashion.
High-throughput data on chromatin distribution in hundreds of samples will be generated, allowing to decipher the pathogenic function of tumor heterogeneities in tumor progression. These data will be used as benchmarks for predicting differential responsiveness and/or resistance of cancer cells to targeted therapies opening brand new possibilities for medical diagnosis and therapeutic guidance.
The consortium is formed by young scientists from Universities in the field of photonics, computer sciences and epigenetics, and a leading company in microfluidics.

Call for proposal

H2020-FETOPEN-2016-2017

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Sub call

H2020-FETOPEN-1-2016-2017

Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Net EU contribution
€ 371 250,00
Address
Piazzale Aldo Moro 7
00185 Roma
Italy

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Region
Centro (IT) Lazio Roma
Activity type
Research Organisations
Non-EU contribution
€ 0,00

Participants (8)

Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

POLITECNICO DI MILANO
Italy
Net EU contribution
€ 230 000,00
Address
Piazza Leonardo Da Vinci 32
20133 Milano

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Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
United Kingdom
Net EU contribution
€ 511 880,00
Address
South Kensington Campus Exhibition Road
SW7 2AZ London

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Region
London Inner London — West Westminster
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
UNIVERSITA DEGLI STUDI DI TRENTO
Italy
Net EU contribution
€ 492 873,75
Address
Via Calepina 14
38122 Trento

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Region
Nord-Est Provincia Autonoma di Trento Trento
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE LYON
France
Net EU contribution
€ 304 000,00
Address
20 Avenue Albert Einstein
69621 Villeurbanne Cedex

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Region
Auvergne-Rhône-Alpes Rhône-Alpes Rhône
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

UNIVERSITE LYON 1 CLAUDE BERNARD
France
Net EU contribution
€ 25 000,00
Address
Boulevard Du 11 Novembre 1918 Num43
69622 Villeurbanne Cedex

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Region
Auvergne-Rhône-Alpes Rhône-Alpes Rhône
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
Net EU contribution
€ 31 250,00
Address
Rue Michel Ange 3
75794 Paris

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
Non-EU contribution
€ 0,00
ELVESYS
France
Net EU contribution
€ 499 271,25
Address
172 Rue De Charonne B2 2 Eme Etage
75011 Paris

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Ile-de-France Ile-de-France Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
UNIVERSITE D'ANGERS
France
Net EU contribution
€ 31 000,00
Address
Rue De Rennes 40
49035 Angers Cedex 01

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Region
Pays de la Loire Pays de la Loire Maine-et-Loire
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00