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Directed Evolution of Novel Artificial Metalloenzyme Platforms for Biocatalysis

Objetivo

Artificial metalloenzymes have emerge as a fusion of synthetic and biological catalysts for environmentally friendly synthesis. These hybrid catalysts are created by tethering a catalytic metal ion or cofactor to a protein scaffold. Optimized artificial metalloenzymes have the potential to harness the advantages of both systems, inheriting the versatility of synthetic catalysts and the efficiency and robustness of enzymes. New tools, however, are required to realize the potential of artificial metalloenzymes. The objective of the ArtMetBio project is to create novel platforms for artificial metalloenzymes that optimize catalytic performance and versatility via increased cooperation between the synthetic and biological components.

Now, more than ever, green catalysis is a crucial global endeavor. Our growing ability to evolve enzymes enables the use of new, unexpected protein scaffolds, opening the door to new innovative platforms. Taking advantage of directed evolution methods, this proposal outlines a strategy for repurposing two well characterized protein scaffolds to create artificial metalloenzymes for in vivo metathesis, a canonical reaction in synthetic chemistry not found in nature. Aim 1 of the project is to optimize metathesis-catalyzing enzymes, coined metathases, through directed evolution. Aim 2 will apply these metathases to engineer a biocatalytic cascade for in vivo synthesis of high-value privileged compounds. The proposed systems differ from the current state-of-the art—streptavidin and heme artificial metalloenzymes—because they are monomeric, non-toxic, and highly modular, enabling wide catalytic versatility. The proposed action has the potential to provide unique, versatile tools for synthesis and biocatalysis.

Régimen de financiación

MSCA-IF-EF-ST - Standard EF

Coordinador

UNIVERSITAT BASEL
Aportación neta de la UEn
€ 175 419,60
Dirección
PETERSPLATZ 1
4051 Basel
Suiza

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Región
Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Stadt
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 175 419,60