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Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

Objective

ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e. the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,

Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Net EU contribution
€ 261 499,68
Address
Piazzale Aldo Moro 7
00185 Roma
Italy

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Region
Centro (IT) Lazio Roma
Activity type
Research Organisations
Non-EU contribution
€ 0,00

Participants (6)

PARIS-LODRON-UNIVERSITAT SALZBURG
Austria
Net EU contribution
€ 132 103,62
Address
Kapitelgasse 4-6
5020 Salzburg

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Region
Westösterreich Salzburg Salzburg und Umgebung
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA
Spain
Net EU contribution
€ 90 604,54
Address
Campus De La Uab Edifici Q Icn2
08193 Bellaterra (Barcelona)

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Region
Este Cataluña Barcelona
Activity type
Research Organisations
Non-EU contribution
€ 0,00
DIAGNOSTICUM GYARTO ES FORGALMAZO ZARTKORUEN MUKODO RESZVENYTARSASAG
Hungary
Net EU contribution
€ 114 857,64
Address
Attila U 126
1047 Budapest

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Közép-Magyarország Budapest Budapest
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
MILTENYI BIOTEC GMBH

Participation ended

Germany
Net EU contribution
€ 0,00
Address
Friedrich Ebert Strasse 68
51429 Bergish Gladbach

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Region
Nordrhein-Westfalen Köln Rheinisch-Bergischer Kreis
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
BLUSENSE DIAGNOSTICS APS
Denmark
Net EU contribution
€ 148 761,00
Address
Fruebjergvej 3
2100 Kobenhavn

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Danmark Hovedstaden Byen København
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
MILTENYI BIOTEC BV & CO KG
Germany
Net EU contribution
€ 252 788,40
Address
Friedrich Ebert Strasse 68
51429 Bergisch Gladbach

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Region
Nordrhein-Westfalen Köln Rheinisch-Bergischer Kreis
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00

Partners (2)

Partner

Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement.

APPLIED NANOPARTICLES SL
Spain
Net EU contribution
€ 0,00
Address
Calle Alaba 88
08018 Barcelona

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Este Cataluña Barcelona
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
Partner

Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement.

ALTA RICERCA E SVILUPPO IN BIOTECNOLOGIE SRLU
Italy
Net EU contribution
€ 0,00
Address
Via Fiorentina 151
53100 Siena

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Centro (IT) Toscana Siena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00