Objective
ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e. the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,
Fields of science
Programme(s)
Funding Scheme
MSCA-ITN-EID - European Industrial DoctoratesCoordinator
00185 Roma
Italy
See on map
Participants (6)
5020 Salzburg
See on map
08193 Bellaterra (Barcelona)
See on map
1047 Budapest
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
Participation ended
51429 Bergish Gladbach
See on map
2100 Kobenhavn
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
51429 Bergisch Gladbach
See on map
Partners (2)
Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement.
08018 Barcelona
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement.
53100 Siena
See on map
The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.