Obiettivo Reading the genome is one thing – finding out how it functions, is something else altogether. The next big challenge to understand gene behaviour is deciphering (a) how the genome is organized in space and (b) how this organization influences its function. Inside Eukaryotic cells, genomic DNA is packed together with proteins into a remarkable structure known as chromatin. Nucleosomes, the building blocks of chromatin, interact with each other to enable high-density packaging. Our understanding of chromatin structure is limited by the lack of ‘close up views’ and molecular-level mechanistic information of how nucleosome interactions are regulated in vivo by many highly coupled factors.InsideChromatin aims to develop a groundbreaking multiscale approach that will push the current limits of realistic computational modelling of in vivo chromatin structure. The vision is to achieve the first multiscale simulation study that describes nucleosome organization inside functionally different kilobase-scale domains, while explicitly accounting for the combination of epigenetic marks, the binding of architectural proteins, and nucleosome remodelling activity that distinguishes each domain. InsideChromatin will integrate atomistic simulations with two levels of coarse-graining and experimental data for validation to understand how nucleosome organization at kilobase scales leads to physical properties at megabase scales. The output from InsideChromatin will bring us closer to the ‘holy grail’ of deciphering the connection between genome characteristics, structure, and function. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencescomputer and information sciencescomputational sciencemultiphysicsnatural sciencesbiological sciencesgeneticsgenomes Parole chiave multiscale modelling and simulation chromatin structure coarse-grained modelling genome structure biomolecular modelling and simulation molecular dynamics Monte Carlo simulations DNA packaging Programma(i) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Argomento(i) ERC-2018-STG - ERC Starting Grant Invito a presentare proposte ERC-2018-STG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-STG - Starting Grant Istituzione ospitante THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Contribution nette de l'UE € 1 490 380,00 Indirizzo TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Regno Unito Mostra sulla mappa Regione East of England East Anglia Cambridgeshire CC Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 1 490 380,00 Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo netto dell'UE Espandi tutto Riduci tutto THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Regno Unito Contribution nette de l'UE € 1 490 380,00 Indirizzo TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Mostra sulla mappa Regione East of England East Anglia Cambridgeshire CC Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 1 490 380,00