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Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Objective

The detection of biomarkers in body fluids is of great importance for disease early diagnosis. The clinical practice uses typically immunoassay methods (e.g. ELISA, Enzyme-Linked Immuno Sorbent Assay, and related techniques) for biomarker determination. Unfortunately, these techniques fail dramatically in a wide variety of cases where the concentration of biomarkers falls below the limit of detection (LOD). The Alzheimer’s disease (AD) is one real example. Nowadays the AD diagnosis from blood collection is not possible just for the above-mentioned reason. The AD biomarkers are low abundant in blood and they can be determined only in cerebrospinal fluid (CSF), thus requiring a highly invasive intervention on the patient (lumbar puncture) under hospitalization, causing also high costs for the public health. SensApp seeks to develop a bench-top “super-sensor” able to determine low abundant AD biomarkers simply in plasma, from peripheral blood, for very early and non-invasive diagnosis in routine clinical practice. We will develop an outstanding innovative technology that we call droplet split-and-stack (DSS), able to stack the biomarker molecules in sub-microlitre volumes on a solid support before the immunoreactions, thus avoiding diffusion limits and improving significantly the LOD. The super-sensor will be fully automated and cost-effective. An integrated micro-system in polar materials will split the mother drop of the plasma sample in tiny droplets through electric fields and will accumulate them on a microscale site, while an innovative integrated optical system will detect the fluorescence signal directly on the reaction support. SensApp will lay the foundations for a future European industrial leadership involved in all of those clinical studies where the detection of low abundant biomarkers is of vital importance for the welfare of society.
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Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE

Address

Piazzale Aldo Moro 7
00185 Roma

Italy

Activity type

Research Organisations

EU Contribution

€ 831 562,50

Participants (5)

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VRIJE UNIVERSITEIT BRUSSEL

Belgium

EU Contribution

€ 654 200

IRCCS CENTRO NEUROLESI BONINO PULEJO

Italy

EU Contribution

€ 124 375

UNIVERSITAT LINZ

Austria

EU Contribution

€ 416 197,50

Teknologian tutkimuskeskus VTT Oy

Finland

EU Contribution

€ 762 477,50

GINOLIS OY

Finland

EU Contribution

€ 498 750

Project information

Grant agreement ID: 829104

Status

Ongoing project

  • Start date

    1 January 2019

  • End date

    31 December 2021

Funded under:

H2020-EU.1.2.1.

  • Overall budget:

    € 3 287 562,50

  • EU contribution

    € 3 287 562,50

Coordinated by:

CONSIGLIO NAZIONALE DELLE RICERCHE

Italy