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Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation

Objective

Enzymatic cascades open a path to the efficient implementation of complex biotransformations for producing from high-cost pharmaceuticals to low-cost biocommodities. However, for multi-step synthetic schemes catalyzed by incompatible or unpaired enzymatic cascades, efficient cell-free one-pot systems, where enzymes are perfectly orchestrated and regulated, have yet to be developed. In HOTZYMES, we propose to develop a new ground-breaking concept to exert functional control over different enzymes using magnetic heating. We expect to control enzyme activity by conjugating enzymes and magnetic nanoparticles and tuning temperature gradients at the nanoscale to precisely and locally reach the optimal temperature of each immobilized enzyme. This will allow an unprecedented spatio-temporal control over the kinetics of multi-enzymatic cascades by remotely applying alternating magnetic fields. To ensure an easy separation, re-utilization and integration into bioreactors, these conjugates will be integrated within porous microparticles. To meet this technological break-through we will need to design and fabricate a new generation of magnetic bioreactors specific to the field of Biocatalysis. We will need to answer fundamental questions about the physical mechanisms that govern heat transfer/diffusion between magnetic materials and biomolecules at different spatial scales by bringing together different scientific and technological disciplines and expertise. HOTZYMES will thus contribute to change current industrial biotransformations from an unsatisfactory current paradigm (uncoordinated enzyme function, sequential reactions, disposable bioprocesses) into a game-changing breakthrough (coordinated enzyme function, concurrent reactions, recyclable bioprocesses).

Field of science

  • /engineering and technology/industrial biotechnology/bioprocessing technologies/biocatalysis
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /engineering and technology/environmental biotechnology/bioremediation/bioreactor

Call for proposal

H2020-FETOPEN-2018-2019-2020-01
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Funding Scheme

RIA - Research and Innovation action

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Address
Calle Serrano 117
28006 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 810 532,50

Participants (7)

FORSCHUNGSZENTRUM JULICH GMBH
Germany
EU contribution
€ 393 821,25
Address
Wilhelm Johnen Strasse
52428 Julich
Activity type
Research Organisations
UNIVERSIDAD DE ZARAGOZA

Participation ended

Spain
EU contribution
€ 18 151,95
Address
Calle Pedro Cerbuna 12
50009 Zaragoza
Activity type
Higher or Secondary Education Establishments
TECHNISCHE UNIVERSITAET GRAZ
Austria
EU contribution
€ 503 110
Address
Rechbauerstrasse 12
8010 Graz
Activity type
Higher or Secondary Education Establishments
UNIVERSITA DEGLI STUDI DELL'INSUBRIA
Italy
EU contribution
€ 250 000
Address
Via Ravasi 2
21100 Varese
Activity type
Higher or Secondary Education Establishments
NANOSCALE BIOMAGNETICS SOCIEDAD LIMITADA
Spain
EU contribution
€ 497 000
Address
Calle Panama 2, Local 1
50012 Zaragoza
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ACIB GMBH
Austria
EU contribution
€ 216 237,50
Address
Krenngasse 37/2
8010 Graz
Activity type
Research Organisations
ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Spain
EU contribution
€ 303 535,55
Address
Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian
Activity type
Research Organisations