CORDIS
EU research results

CORDIS

English EN
Next generation precision antibody profiling - from science fiction to reality

Next generation precision antibody profiling - from science fiction to reality

Objective

One of the major challenges of modern medicine is to understand how the human organism defends itself against invasions and diseases. The biggest mystery is the human immune system, and understanding this ultimately requires knowledge of the sequence repertoire of human antibodies and their respective antigens. The purpose of the TopSpec project is to be the first in the world to solve this challenge, opening up opportunities in medical research and drug development that are today only dreamt about. We will create a breakthrough technology that will revolutionize academic, clinical and industrial proteomics and dramatically advance the development of new generation antibody- and protein-based therapeutics. Antibodies represent the most sophisticated line of natural defense against disease. Knowing exactly which antibodies are produced in response to a particular disease enables us not only to better understand the cause of the disease but also to provide new-generation cures in the form of personalized therapeutic antibodies. The limiting factor for this to truly be achieved is to find a way to analyze and sequence large molecules in the gas phase, and this represents a formidable challenge. The TopSpec project will develop ground-breaking TOP-down tandem mass SPECtrometry (MS/MS) approaches based on novel radical gas-phase ion-electron and ion-atom reactions, and implement them on a unique, hyphenated, ultrahigh-resolution MS platform. Another “killer innovation” is the ability to greatly simplify MS/MS spectra of large molecules by adding another dimension of separation – collisional cross-sections of fragment ions using two parallel approaches. TopSpec will be the first project to implement de-convolution of massively overlapping isotopic clusters, solving one of the greatest challenges in top-down MS of large molecules.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

KAROLINSKA INSTITUTET

Address

Nobels Vag 5
17177 Stockholm

Sweden

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 989 500

Participants (7)

Sort alphabetically

Sort by EU Contribution

Expand all

FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA

Greece

EU Contribution

€ 860 500

THERMO FISHER SCIENTIFIC (BREMEN) GMBH

Germany

EU Contribution

€ 695 500

SPECTROSWISS SARL

Switzerland

EU Contribution

€ 430 500

BIOMOTIF AB

Sweden

EU Contribution

€ 325 000

THE NOTTINGHAM TRENT UNIVERSITY

United Kingdom

EU Contribution

€ 306 500

INSTITUT PASTEUR

France

EU Contribution

€ 278 500

SPECTROMETRY VISION BV

Netherlands

EU Contribution

€ 105 500

Project information

Grant agreement ID: 829157

Status

Ongoing project

  • Start date

    1 January 2019

  • End date

    31 December 2021

Funded under:

H2020-EU.1.2.1.

  • Overall budget:

    € 3 991 500

  • EU contribution

    € 3 991 500

Coordinated by:

KAROLINSKA INSTITUTET

Sweden