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Site-specific Ultrasensitive Magnetic resonance of Mixtures for Isotopic Tracking

Objective

There is a high demand to design approaches capable of tracking the origin of biomarkers in complex biological environments, in the areas of life, environmental, food and forensic sciences. Metabolomics and Fluxomics show great promises towards this aim, and a high potential arises from their combination with Isotopic fingerprinting at natural abundance. The resulting isotopomics approach requires cutting-edge analytical tools, and Nuclear Magnetic Resonance (NMR) is currently the only generic technique giving access to the site-specific isotope content at natural abundance. The detection of very small relative variations between samples originating from different (bio)chemical pathways is possible through 13C isotopic NMR, which can however only be applied to simple and concentrated samples, due to its low sensitivity. Consequently, numerous applications are out of reach. To tackle the current limitations of 13C isotopic analysis, SUMMIT will develop a groundbreaking analytical workflow relying on two of the most powerful NMR methods: dissolution dynamic nuclear polarization and ultrafast 2D NMR. This cutting-edge approach will allow the simultaneous measurement of 13C fingerprints from multiple low-concentrated biomarkers in complex mixtures, which is impossible with existing methods. The high potential of this analytical strategy will be demonstrated on a relevant biological study, the investigation of breast cancer cell metabolism, through applications with gradually increasing risk levels. These approaches will make it possible to identify (i) new biomarkers to discriminate between cell lines expressing different hormonal receptors; (ii) novel potential therapeutic targets from the elucidation of metabolic pathways. Beyond this application, the project will have a high impact on a wide community of academic and industrial researchers, covering unmet needs from life sciences, food industry and forensic analysis.

Host institution

UNIVERSITE DE NANTES

Address

Quai De Tourville 1
44035 Nantes Cedex 1

France

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 999 768

Beneficiaries (1)

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UNIVERSITE DE NANTES

France

EU Contribution

€ 1 999 768

Project information

Grant agreement ID: 814747

Status

Ongoing project

  • Start date

    1 October 2019

  • End date

    30 September 2024

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 999 768

  • EU contribution

    € 1 999 768

Hosted by:

UNIVERSITE DE NANTES

France