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Synthetic biology-guided engineering of Pseudomonas putida for biofluorination

Synthetic biology-guided engineering of Pseudomonas putida for biofluorination

Objective

Nature has hardly evolved biochemical reactions involving fluorine (F), the most abundant halogen on Earth. Organic compounds containing F (fluorochemicals) are, however, extremely relevant from an industrial point of view. Fluoropolymers are the main fluorochemicals in the market worldwide, and are exclusively synthesized using chemical methods. Moreover, current fluorination technologies usually involve corrosive and toxic reagents that have a negative impact on the environment. Designing sustainable bioprocesses based on alternative and safer fluorinating agents from renewable substrates is thus a long-sought-after, yet unfulfilled goal. SinFonia proposes to engineer the metabolically-versatile bacterium Pseudomonas putida to execute biofluorinations for generating novel fluoropolymers from renewable substrates. P. putida KT2440, a non-pathogenic soil bacterium, serves as an ideal microbial platform for F-dependent biochemical reactions due to its extraordinary resistance to harsh and stressful operating conditions. SinFonia will exploit natural selection to enhance bioproduction through a smart strain engineering approach in which bacterial growth will be coupled to biofluorination. Our target compounds are a whole family of fluorinated polyesters with enhanced physicochemical and material properties, with uses as self-cleaning surfaces, low-surface-energy coatings, bio-based lubricants, membranes for fuel cells, and anti-fouling materials. The versatile P. putida strains engineered during the project can be easily adapted to synthesize other added-value fluorochemicals. Unlike chemical processes, the source of F in our system will be NaF, an inexpensive and safe salt, and sugars as the main carbon source. In-depth analysis of all the environmental and economic benefits of the new fluorination technology, and interactive communication of social benefits associated with target products, are essential components of SinFonia.

Coordinator

DANMARKS TEKNISKE UNIVERSITET

Address

Anker Engelundsvej 1 Bygning 101 A
2800 Kgs Lyngby

Denmark

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 811 921,39

Participants (12)

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MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

Germany

EU Contribution

€ 1 010 875

Masarykova univerzita

Czechia

EU Contribution

€ 435 287,50

THE UNIVERSITY COURT OF THE UNIVERSITY OF ST ANDREWS

United Kingdom

EU Contribution

€ 481 250

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS

Spain

EU Contribution

€ 443 965

TARTU ULIKOOL

Estonia

EU Contribution

€ 390 000

UNIVERSITE DU LUXEMBOURG

Luxembourg

EU Contribution

€ 631 590

BIOPLASTECH LTD

Ireland

EU Contribution

€ 715 103,75

BIOFACTION KG

Austria

EU Contribution

€ 375 268,75

ALTAR

France

EU Contribution

€ 302 560

IN SRL

Italy

EU Contribution

€ 382 682,09

IFEU - INSTITUT FUR ENERGIE UND UMWELTFORSCHUNG HEIDELBERG GMBH

Germany

EU Contribution

€ 373 000

CHEMOURS NETHERLANDS BV

Netherlands

EU Contribution

€ 595 000

Project information

Grant agreement ID: 814418

Status

Ongoing project

  • Start date

    1 January 2019

  • End date

    28 February 2023

Funded under:

H2020-EU.2.1.4.

  • Overall budget:

    € 7 948 503,48

  • EU contribution

    € 7 948 503,48

Coordinated by:

DANMARKS TEKNISKE UNIVERSITET

Denmark