Synthesis of proteins with on-demand activation for real-time in-cell studies
To monitor proteins and their cellular functions in real time, the protein synthesis and in situ protein manipulation has to be accompanied by advanced methods for protein delivery and real-time activation. The EU-funded SynProAtCell project will develop proteins with traceless cell delivery units, an on-demand function activation unit, and a fluorescence probe for monitoring. The goal is to use this approach to elucidate the processes of ubiquitination and deubiquitination, the critical regulators for many biological processes. The project will study the effect of inhibition of deubiquitinases in cancer, the effect of phosphorylation on proteasomal degradation and ubiquitin chain elongation, and the effect of covalent attachment of a ligase ligand to a protein on its degradation.
Fields of science
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