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Single molecule sequencing and biophysical properties of oxidized genomic DNA using magnetic tweezers.

Project description

Scissors and tweezers may soon lead the pack of advanced DNA sequencing methods

DNA undergoes numerous biochemical and biophysical changes to modulate gene expression. These epigenetic modifications do not alter the DNA sequence but can affect how it is 'read'. A common example is methylation (addition of CH3), which physically impedes transcription and gene expression. While synthetic chemistry and DNA sequencing techniques have advanced tremendously, they do not preserve critically valuable epigenetic information. The EU-funded EpiSeq project is using high-tech 'molecular scissors' (CRISPR/Cas9 technology) to cut out the small sequence of DNA of interest, and magnetic tweezers to manipulate the molecule and determine its epigenetic sequence. Knowledge of epigenetic changes will inform studies on how gene expression changes cause certain diseases with implications for better diagnoses and treatment.

Coordinator

MUSEUM NATIONAL D'HISTOIRE NATURELLE
Net EU contribution
€ 184 707,84
Address
Rue Cuvier 57
75005 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Higher or Secondary Education Establishments
Other funding
€ 0,00