Improved light-sheet microscopy prototype enables a better view of cell dynamics
Light-sheet fluorescence microscopy (LSFM) is a technique in which a very thin slice of a sample is illuminated by a laser source perpendicularly to the direction of observation. The EU-funded MAFIn project developed a universally compatible module capable of fast volumetric imaging that combines LSFM with waveform coding. The latter introduces a phase mask in the detection arm of the LSFM platform to extend the depth of field. A deconvolution operation needs to be applied as the phase mask inevitably distorts the obtained images. The project provided a calibration procedure with optimised deconvolution strategies so that LSFM with wavefront coding can be performed in a simple way. The new prototype holds great promise for higher-resolution imaging of cell dynamics.
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