Skip to main content

Module for Aberration Correction and Fast Volumetric Imaging in a Light Sheet Fluorescence Microscope

Project description

Improved light-sheet microscopy prototype enables a better view of cell dynamics

Light-sheet fluorescence microscopy (LSFM) is a technique in which a very thin slice of a sample is illuminated by a laser source perpendicularly to the direction of observation. The EU-funded MAFIn project developed a universally compatible module capable of fast volumetric imaging that combines LSFM with waveform coding. The latter introduces a phase mask in the detection arm of the LSFM platform to extend the depth of field. A deconvolution operation needs to be applied as the phase mask inevitably distorts the obtained images. The project provided a calibration procedure with optimised deconvolution strategies so that LSFM with wavefront coding can be performed in a simple way. The new prototype holds great promise for higher-resolution imaging of cell dynamics.

Call for proposal

H2020-FETOPEN-2018-2020

See other projects for this call

Sub call

H2020-FETOPEN-2018-2019-2020-03

Coordinator

FUNDACIO INSTITUT DE CIENCIES FOTONIQUES
Net EU contribution
€ 100 000,00
Address
Avinguda Carl Friedrich Gauss 3
08860 Castelldefels
Spain

See on map

Region
Este Cataluña Barcelona
Activity type
Research Organisations
Other funding
€ 100 000,00