CORDIS
EU research results

CORDIS

English EN

Signaling dynamics in the control of cell proliferation and differentiation during development and homeostasis

Objective

Tight coordination between proliferation and differentiation is key to proper development and homeostasis of multicellular systems. Cell-cell communication via signaling pathways has long been studied in this context. Encoding information in the temporal change of a signal, i.e. signaling dynamics, can ensure information transmission to be specific and accurate. Studies exemplifying the relevance of dynamic signaling raise the critical question: What is the function of signaling dynamics in controlling proliferation and differentiation at tissue level? My expertise in signaling dynamics, advanced light microscopy, microfluidics, development, cell cycle research and organoid culture provides a unique skill-set for the functional investigation of dynamic signaling during development and tissue homeostasis using two model systems:

Somitogenesis in vertebrate embryos is the sequential segmentation of growing tissue. It is controlled by signaling gradients and oscillations. Whereas differentiation has been studied extensively, it remains elusive (A) how cell proliferation is regulated and (B) whether there is a link between proliferation and signaling dynamics. Indeed, our preliminary data strongly indicate that cell proliferation impacts on signaling oscillations. Homeostasis of adult tissue is also maintained by signaling pathways balancing proliferation and differentiation. In the small intestine some of these pathways have recently been shown to be dynamic. We will apply a systematic approach to understand (A) are signaling pathways in the intestine dynamic and (B) what is the function. Our preliminary data support that combining organoid culture, light-sheet microscopy and microfluidic perturbation allows quantification and functional analysis of signaling dynamics.

By comparing signaling dynamics in development and homeostasis we will derive general principles of dynamic signal encoding in multicellular systems.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Host institution

KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW

Address

Kloveniersburgwal 29 Het Trippenhuis
1011 Jv Amsterdam

Netherlands

Activity type

Other

EU Contribution

€ 1 500 000

Beneficiaries (1)

Sort alphabetically

Sort by EU Contribution

Expand all

KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW

Netherlands

EU Contribution

€ 1 500 000

Project information

Grant agreement ID: 850554

Status

Grant agreement signed

  • Start date

    1 March 2020

  • End date

    28 February 2025

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 500 000

  • EU contribution

    € 1 500 000

Hosted by:

KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW

Netherlands