CORDIS
EU research results

CORDIS

English EN

Dissecting the chromatin response to DNA damage in silenced heterochromatin regions

Project information

Grant agreement ID: 850405

Status

Ongoing project

  • Start date

    1 December 2019

  • End date

    30 November 2024

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 499 404

  • EU contribution

    € 1 499 404

Hosted by:

UNIVERSITAIR MEDISCH CENTRUM UTRECHT

Netherlands

Objective

Cells are continuously exposed to insults that can break or chemically modify their DNA. To protect the DNA, cells have acquired an arsenal of repair mechanisms. Proper repair of DNA damage is essential for organismal viability and disease prevention. What is often overlooked is the fact that the eukaryotic nucleus contains many different chromatin domains that can each influence the dynamic response to DNA damage. Different chromatin environments are defined by specific molecular and biophysical properties, which could necessitate distinct chromatin responses to ensure safe DNA damage repair.
The aim of this proposal is to understand how diverse chromatin domains, and in particular the dense heterochromatin environment, shape the dynamic chromatin response to DNA damage.
I recently developed locus-specific DNA damage systems that allow for in-depth analysis of chromatin domain-specific repair responses in Drosophila tissue. I will employ these systems and develop new ones to directly observe heterochromatin-specific dynamics and repair responses. I will combine these systems and state-of-the art chromatin analysis with high-resolution live imaging to dissect the DNA damage-associated heterochromatin changes to determine their function in repair -kinetics, -dynamics and -pathway choice.
Deciphering the chromatin dynamics that regulate DNA damage repair in heterochromatin will have broad conceptual implications for understanding the role of these dynamics in other essential nuclear processes, such as replication and transcription. More importantly, understanding how chromatin proteins promote repair will be important in determining how cancer-associated mutations in these chromatin proteins impact genetic instability in tumours in the long run.

Host institution

UNIVERSITAIR MEDISCH CENTRUM UTRECHT

Address

Heidelberglaan 100
3584 Cx Utrecht

Netherlands

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 499 404

Beneficiaries (1)

UNIVERSITAIR MEDISCH CENTRUM UTRECHT

Netherlands

EU Contribution

€ 1 499 404

Project information

Grant agreement ID: 850405

Status

Ongoing project

  • Start date

    1 December 2019

  • End date

    30 November 2024

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 499 404

  • EU contribution

    € 1 499 404

Hosted by:

UNIVERSITAIR MEDISCH CENTRUM UTRECHT

Netherlands