Gene regulation and function of the 3D genome
The three-dimensional organisation of the genome inside the nucleus, referred to as the 3D genome (3DG), is crucial for gene regulation. The building blocks of the 3DG have been identified, but their contribution to gene regulation is not clear. The EU-funded FuncDis3D project aims to functionally dissect the 3DG to understand its effects on gene expression. The approach is to induce changes in the 3DG by acute protein depletion and evaluate these changes over time. The preliminary data show that by depleting regulators of the 3DG the changes occur within a few hours. Researchers will deplete a range of proteins in pluripotent and differentiated cells to determine their contribution to 3DG organisation. Simultaneous characterisation of the epigenome and transcriptome will allow them to determine the sequence of changes.
Fields of science
Funding SchemeERC-COG - Consolidator Grant
1066 CX Amsterdam
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