Mass cytometry nanotools for intracellular protein targeting
The discovery of multi-kinase inhibitors has emerged as a strong novel treatment for many human cancers. However, the severe side effects due to their intrinsic off-target action impose restrictions on their dosage and limit their efficacy. The EU-funded TARGETOF project proposes an efficient approach to detect the binding of a given drug to intracellular target proteins. The approach combines intracellular target engagement devices based on nanotechnology with state-of-the-art mass cytometry techniques, allowing one-step target engagement profiling, thus streamlining the discovery of the action profile of a given drug. This research will greatly contribute to the development of straightforward methods to detect the most expressed targets in the biopsies and select the best course of treatment for the patient.