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Single molecule protein sequencing using biological nanopores

Objective

Despite the importance of post-translational variation to the function of proteins in a living organism, sequencing proteins to study these variations is still a costly and time-consuming process, with intrinsic limitations. Protein sequencing and detection of post-translational modifications (PTMs) by mass spectrometry, the current gold standard, requires extensive sample preparation and large sample sizes, and possesses a limited dynamic range with respect to sample concentration. These limitations severely restrict its application to biological and clinical problems. A robust method for sequencing proteins and detecting PTMs at the single-molecule level would be revolutionary for proteomics research, allowing biologists to quantify low-abundance proteins as well as distributions and correlations of PTM patterns, all at a single-cell level. I propose a first-of-kind method for protein sequencing with applications in fundamental biology, cancer immunotherapy, and pharmaceutical development. This method uses biological nanopores in a manner similar to nanopore DNA sequencing, an established single-molecule sequencing technology capable of high throughput and single-molecule sensitivity. Developing this method for protein sequencing comes with many significant challenges, which will be addressed over the course of the proposed research.

Call for proposal

H2020-MSCA-IF-2019
See other projects for this call

Funding Scheme

MSCA-IF-EF-ST - Standard EF

Coordinator

TECHNISCHE UNIVERSITEIT DELFT
Address
Stevinweg 1
2628 CN Delft
Netherlands
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 187 572,48