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Functional and structural studies of the U12-dependent splicing in human cells

Project description

Characterisation of the minor spliceosome pathway in human cells

Introns are non-coding segments of eukaryotic genes removed from mRNA precursors by the spliceosome. In human cells, most of the introns are processed by the U2-dependent spliceosome. However, around 0.5 % of human introns are processed by the minor spliceosome, which depends on the U12 small nuclear RNA. These introns are often located in genes with critical cellular functions, and mutations in minor spliceosome components lead to genetic disorders. The EU-funded MinorSplice project will work towards the comprehensive structural and functional characterisation of the U12-dependent splicing pathway. A combination of cell-based assays, proteomics and next-generation sequencing methods will provide detailed information about minor spliceosome composition and related regulatory mechanisms.

Host institution

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Net EU contribution
€ 1 479 277,00
Address
Meyerhofstrasse 1
69117 Heidelberg
Germany

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Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Research Organisations
Other funding
€ 1 479 277,00

Beneficiaries (1)

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
Net EU contribution
€ 1 479 277,00
Address
Meyerhofstrasse 1
69117 Heidelberg

See on map

Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Research Organisations
Other funding
€ 1 479 277,00