Objetivo To modify selected pyridoxal phosphate-dependent enzymes by site directed mutagenesis in order to produce new catalytic capabilities and to alter susceptibility to mechanism-based inhibitors. The programme makes use of the well-studied aspartate aminotransferase as a prototypic enzyme for which much structural and kinetic information is already available and of thermostable forms of this enzyme from high temperature organisms. Glutamate semialdehyde aminotransferase and glutamate decarboxylase are also under investigation and the solution of the structures of these enzymes by X-ray crystallography is an associated objective.Two mutants of aspartate aminotransferase from Escherichia coli have been produced, one of which has been endowed with alanine racemase activity and the other with b-decarboxylase activity towards L-aspartate. Other mutants have been produced in which the partition ratio between b-elimination and transamination and inactivation have been extensively altered. The structure of glutamate semialdehyde aminotransferase has been solved and mutant forms of the enzyme produced accordingly. The two glutamate decarboxylase genes in E. coli have been separately expressed and the effect of mutating active site histidines has been determined. A mutant of the thermophilic aspartate aminotransferase from Sulfolobus solfataricus enzyme has been created in which a temperature dependent conformational change has been analysed fluorometrically. Ámbito científico natural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural scienceschemical sciencescatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP3-HCM - Specific research and technological development programme (EEC) in the field of human capital and mobility, 1990-1994 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador University of Wales, Cardiff Aportación de la UE Sin datos Dirección CF1 1ST Cardiff Reino Unido Ver en el mapa Coste total Sin datos Participantes (7) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH Alemania Aportación de la UE Sin datos Dirección Correnstrasse 3 06466 GATERSLEBEN Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II Italia Aportación de la UE Sin datos Dirección Via Mezzocannone 16 80134 NAPOLI Ver en el mapa Coste total Sin datos University of East London Reino Unido Aportación de la UE Sin datos Dirección Romford Road E15 4LZ London Ver en el mapa Coste total Sin datos University of Hertfordshire Reino Unido Aportación de la UE Sin datos Dirección Hatfield Campus College Lane AL10 9AB Hatfield Ver en el mapa Coste total Sin datos Universitá degli Studi di Roma 'La Sapienza' Italia Aportación de la UE Sin datos Dirección Piazzale Aldo Moro 00185 Roma Ver en el mapa Coste total Sin datos Universität Basel Suiza Aportación de la UE Sin datos Dirección Klingeelbergstraße 4056 Basel Ver en el mapa Coste total Sin datos Universität Zürich Suiza Aportación de la UE Sin datos Dirección 190,Winterhurerstraße 8057 Zürich Ver en el mapa Coste total Sin datos
