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Contenu archivé le 2022-12-23

Radiation-induced processes in mammalian cells: principles of response modification and involvement in carcinogenesis

Objectif



We will use a novel quantitative approach (Lalev, Anaschkova, Russev, Eur.J.Biochem. in press) to study the effects of gamma-irradiation or the different stages of DNA synthesis in eukaryotic cells.
We will use two cell lines - Ehrlich-Lettre ascites tumor cells and Friend transformed erythroid cells, that could be regarded as standing at the opposite ends of the spectrum in respect to their ability to control the cell cycle and differentiate.We will follow the kinetics of initiation of the synthesis of both genomic and mitochondrial DNA after exposure to 60Co to obtain information about the mechanism of inhibition of DNA synthesis by gamma-irradiation.

An attempt will made to determine the chromatin structure in the regions of initiation of DNA synthesis and attachment to nuclear matrix in norma and after irradiation.
To this end nuclei will be digested with micrococcal nuclease and DNAse I, DNA will be additionally digested with restriction enzymes and will be hybridized to in vitro labelled DNA prob
and other functional elements.
In a parallel series of experiments we will study the repair kinetics of different transcribed and non-transcribed genes after gamma-irradiation. To achieve this we will use the recently developed universal method for determination of the rates of DNA repair of different genes (Russev, Boulikes, Eur.J.Biochem. 204, 267, 1992).

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

Rijksuniversiteit Leiden
Contribution de l’UE
Aucune donnée
Adresse
72,Wassenaarseweg
2300 AL Leiden
Pays-Bas

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Coût total
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Participants (1)