Cel Mutants defective in DNA double strand break (dsb) rejoining have been isolated from bacteria, lower eukaryotes and mammalian cells, and all show a major sensitivity to ionising radiation (IR) with little cross sensitivity to UV radiation. All these mutants from lower organisms display a defect in recombination, and the aim of this project is to investigate whether a similar relationship between dsb rejoining and recombination exists in mammalian mutants. Homologous recombination will be examined between markers which have been integrated intrachromosomally. For this purpose a construct bearing two copies of a dominant selectable gene (neo), each containing a non-overlapping deletion, will be used. The construct will also contain a second dominant selectable gene (Ecogpt), which will enable stable transfectants bearing the two non-functional-neo genes to be isolated from the cell line under study. Subsequent selection-for a functional neo gene (clones resistant to the antibiotic G418) will provide a measure of the homologous recombination frequency. Both the spontaneous and radiation induced recombination frequencies will be examined. This study will be initially carried out using the xrs mutants which are members of ionising radiation complementation group 5. This line is particularly useful since revertants can be easily isolated following treatment with azacytidine. Thus, a pair of cell lines, phenotypically xrs- and xrs+ can be derived, in which the construct is integrated at the same location. If initial studies with the xrs cells prove that this is a useful system for measuring homologous recommenation, then further studies will be undertaken with the radiosensitive mouse line, L5178Y/s. Program(-y) IC-PECO/COPERNICUS - Scientific and technological cooperation between the European Community and European non-member countries, 1992- Temat(-y) 0501 - CEEC participation in NUCLEAR FISSION SAFETY programme (RADIATION PROTECTION) Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator Rijksuniversiteit Leiden Wkład UE Brak danych Adres 72,Wassenaarseweg 2300 RA Leiden Niderlandy Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (2) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Institute of Nuclear Chemistry and Technology Polska Wkład UE Brak danych Adres 16,ul. Dorodna 03 195 Warszawa Zobacz na mapie Koszt całkowity Brak danych Warsaw Medical School Polska Wkład UE Brak danych Adres 1,ul. Banacha 02 097 Warszawa Zobacz na mapie Koszt całkowity Brak danych
