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Contenuto archiviato il 2022-12-23

Characterization of genes required for progression through mitosis

Obiettivo



The objectives of this application are to incorporate two Hungarian laboratories into an existing Human Mobility Network to study genes that regulate progression through the cell cycle, utilising Drosophila and fission yeasts as model organisms. The existing West European Network will generate mutants defective in cell division, study their phenotypes, and devise means for the molecular cloning of selected genes. The Hungarian laboratories offer two exciting contributions to this Network in the form of collections of Drosophila mutants, a large number of which affect cell cycle progression. professor szabad's laboratory has established a collection of dominant female sterile mutants, from which only a few mutant loci have been characterised. These lead to abnormal embryonic development, in many cases because of the maternal contribution of an abnorml protein essential for either meiosis or mitosis. It is proposed to study the phenotype of double mutant combinations between these mutants and others known to affect this developmental stage in an attempt to establish regulatory hierarchies. Selected loci will be selected for cloning and further molecular studies. Dr Kiss' laboratory has established a collection of more than 2700 lethal and semi-lethal mutants resulting from the insertion of single p-transposons. Already some 20 mitotic mutants have been identified in this collection. It is proposed to carry out a detailed study of the mitotic defects in these mutants, and to undertake the molecular cloning and characterisation of several of these loci. The research groups in the Network will exchange reagents and mutants, and their combined activities will better our understanding of cell cycle regulation. Genes that regulate the progression through the cell cycle have been highly conserved through evolution. We therefore expect that in the long term our findings will be generally applicable to a wide variety of human diseases in which cell division occurs abnormally, including cancer.

Invito a presentare proposte

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Meccanismo di finanziamento

CSC - Cost-sharing contracts

Coordinatore

University of Dundee
Contributo UE
Nessun dato
Indirizzo

DD1 4HN Dundee
Regno Unito

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Costo totale
Nessun dato

Partecipanti (2)