Objectif - Isolation and characterisation of lactic acid bacteria and its contaminants in Ethiopian and Egyptian native products using classical methods and molecular biological methods;- Study of the biochemical and physiological properties of the lactic acid bacteria aimed especially at the progressive dairy technology;- Composition of the new strains for starter cultures of excellent properties regarding lactic acid bacteria of high bacteriophage resistance, acidification, taste and consistence of the product;- Development of new sorts of cheeses of high product quality as a contribution for the nourishment of a growing population.Expected Outcome- Product improvement and modification of African cheeses developed using African lactic acid bacteria with the exception of their "own", i.e. a new Ayib cheese;- Strains of lactic acid bacteria as an alternative to genetic engineering by obtaining strains with desirable properties of taste and flavour, phage resistance and salt tolerance;- Appropriate stabile strains with salt tolerance especially for a new Domiati cheese;- Knowledge about the phage-host relations of lactic acid bacteria.- Investigations of lactic acid bacteria (lactococci, lactobacilli) in relation to their properties for starter cultures;- Characterisation of the new strains in the phylogenic system using classical (i.e. api system biomérieux) and molecular biological methods (SDS, IEF-gel electrophoresis, 16rRNA);- Biochemical and physiological characterisation of the new strains lactose metabolism, lactose uptake rate of acid production, autolysis, proteolytic activity, salt tolerance, bacteriocines, antibiotic resistance and antibiotic activities;- Investigation of phage-host relations with regard to the phage resistance;- Development of appropriate starter cultures for cheese-making using the new strains;- Investigation of the association with undesired microbes, i.e. pathogenic microbes and their antibiotic resistance. Champ scientifique natural scienceschemical sciencesorganic chemistryorganic acidsnatural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesmedical biotechnologygenetic engineeringmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibioticsmedical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance Programme(s) FP3-STD 3 - Specific research and technological development programme (EEC) in the field of the life sciences and technologies for developing countries, 1990-1994 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Universität Hohenheim Contribution de l’UE Aucune donnée Adresse Garbenstraße 30 70599 Stuttgart Allemagne Voir sur la carte Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Ethiopian Livestock Meat Corporation Éthiopie Contribution de l’UE Aucune donnée Adresse Addis-Abeba Voir sur la carte Coût total Aucune donnée University College Cork Irlande Contribution de l’UE Aucune donnée Adresse Western Road 30 Cork Voir sur la carte Coût total Aucune donnée University of Alexandria Égypte Contribution de l’UE Aucune donnée Adresse Aflaton Street Alexandria Voir sur la carte Coût total Aucune donnée
