Cel - To characterise antigens for an early diagnosis of three endoparasites of ruminants : Fasciola hepatica, Hypoderma bovis and Oestrus ovis;- To produce these antigens as pure as possible to develop kits of immunodiagnostics;- To set up a field immuno-test;- To start an immuno-epidemiological survey of each of these parasites.- In this project, five teams from Algeria, France, Mexico and Portugal have collaborated to develop new tools of field diagnosis for three zoonotic parasitic diseases, fasciolosis, hypodermosis and oestrosis, presenting economical incidences for the livestock productions and public health problems.- Antigens for immunological diagnosis have been characterised for their specificity by experimental infestations with these parasites and experimental infestations with parasites found in the same biotope.- Methods of production of specific semi-purified ones and recombinant proteins for F. hepatica and H. lineatum are described. Field immunological diagnosis technics of low cost have been started (ELISA and DIG-ELISA). Methodology of sampling (sera or milk, individual or pooled) at period defined by the biological cycle of the parasite in each country has also been established. These means of investigation for these three diseases have conducted our groups to develop large immuno-epidemiological surveys. Where the studies were undertaken, therapeutic or biological measures of control have been suggested to the farmes or to the veterinary services and advertisements to the medical doctors.- Nevertheless, if the members of this group have clearly stated the mode of interpretation of these immunological results, training of public and animal health technician and practicians need to be developed to interpret the meaning, the advantages and the limits of utilisation of such technics.- Definition of the antigenic pattern of each parasite, using sequential sampling or sera, following experimental monoinfestation, by western blotting. Analysis of antigenic community between these parasites;- Characterisation of the kinetics of the antibodies during a natural infestation and effect of a chimiotherapeutic treatment on kinetics will be studied by ELISA;- Antigen production will be dependant on the work previously done on each parasite. Liquid chromatography, monoclonal antibodies or molecular biology will be used to produce the protein;- Depending on the field technique chosen, the antigen concentration, serum dilution and condition of use of the other reagents will be ascertained;- In order to use these fields tests in optimum conditions, monthly blood samplings will be carried out during the first two years of the programme, to determine the ideal period to start an immuno epidemiological survey of each disease;- During the last year of the programme, a large epidemiological survey will be carried out with the developed test by each participating group. Dziedzina nauki medical and health scienceshealth sciencespublic healthnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsagricultural sciencesanimal and dairy sciencedomestic animalsanimal husbandrynatural sciencesbiological sciencesmolecular biology Program(-y) FP3-STD 3 - Specific research and technological development programme (EEC) in the field of the life sciences and technologies for developing countries, 1990-1994 Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator Institut National de la Recherche Agronomique (INRA) Wkład UE Brak danych Adres Domaine de l'Orfrasière Monnaie 37380 Nouzilly Francja Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (5) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Ecole Nationale Vétérinaire Algieria Wkład UE Brak danych Adres Avenue Pasteur 16200 El-Harrach (Alger) Zobacz na mapie Koszt całkowity Brak danych Instituto Nacional de InvestigacionesForestales y Agricolas Pecuarias Meksyk Wkład UE Brak danych Adres Carretera Federal Cuernavaca KM 11.5 62500 Cuautla - Cuernavaca-Estado de Morelos Zobacz na mapie Koszt całkowity Brak danych Instituto Nacional de Saude Portugalia Wkład UE Brak danych Adres Av.Padre Cruz 1699 Lisboa Zobacz na mapie Koszt całkowity Brak danych Universidad de Santiago de Compostela Hiszpania Wkład UE Brak danych Adres 81,Avenida de Madrid 27002 Lugo Zobacz na mapie Koszt całkowity Brak danych École Nationale Vétérinaire de Nantes Francja Wkład UE Brak danych Adres Route de Gachet 44087 Nantes Zobacz na mapie Koszt całkowity Brak danych