Obiettivo 1) Generation of chromosome specific DNA libraries for mouse and evaluate chemically induced translocations by fluorescent in situ hybridization in somatic and germ cells.2) To study the influence of telomeric repeat sequences on the formation of chromosomal aberrations in somatic and germ cells of mice.Mouse chromosome specific DNA libraries will be generated by flow sorting specific chromosomes from animals carrying Robertsonian translocations, isolating DNA and amplifying that by linker-adapter PCR technique. These probes will be used to paint specific chromosomes by FISH technique. The frequencies of translocations involving these specific chromosomes in bone marrow as well as mouse spermatogonial cells following in vivo treatment with ENU, MMS, mitomycin C, cyclophosphamide, bleomycin and 6 mercaptopurine will be evaluated.FISH technique with telomere specific repeats will be employed to check the influence of these repeats on formation of chemically induced chromosomal aberrations, especially in germ cells, where it is known that these sequences are amplified. Campo scientifico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticschromosomes Programma(i) FP3-ENV 1C - Specific research and technological development programme (EEC) in the field of the environment, 1990-1994 Argomento(i) 040204 - Risks to health and the environment from chemical substances Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore Rijksuniversiteit Leiden Contributo UE Nessun dato Indirizzo 72,Wassenaarseweg 2300 RA Leiden Paesi Bassi Mostra sulla mappa Costo totale Nessun dato Partecipanti (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITÀ DEGLI STUDI DELLA TUSCIA Italia Contributo UE Nessun dato Indirizzo Via S. Camillo de Lellis 01100 VITERBO Mostra sulla mappa Costo totale Nessun dato
