Objectif 1) To study the metabolism of inorganic arsenic in a population in Argentina heavily exposed to As in drinking water.2) To evaluate the mechanism(s) by which As interferes with DNA repair.3) To evaluate the influence of As on the yield of sister chromatid exchanges and chromosomal aberrations in human lymphocytes exposed to chemical mutagens or UV-B.A group of individuals exposed to inorganic arsenic in drinking water from the Salta province will be investigated to evaluate its metabolism in human. Blood, plasma, urine, nail and hair follicles will be collected and the content of As and its metabolites will be estimated. Blood samples from these individuals will be processed for cytogenetic investigations.The basis for the potentiating effects of inorganic arsenic on the mutagenic effects of chemicals/UV will be elucidated by carrying out DNA repair studies as well as cytogenetic studies. In DNA repair studies, cell lines known to be specifically deficient in certain repair steps will be used. In addition, an in vitro repair assay on plasmids carrying specific lesions in their DNA and using cell extracts in the presence or absence of As, or cell extracts from As treated cells will be used.The influence of As on the yield of chromosomal aberrations and SCEs in human lymphocytes or fibroblasts induced by diverse chemicals with different modes of action or UV-B will be investigated. Chromosome painting using FISH technique will be used to detect translocations in these experiments. Champ scientifique engineering and technologyenvironmental engineeringwater treatment processesdrinking water treatment processeshumanitiesartsvisual artsnatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesinorganic chemistrymetalloidsnatural sciencesbiological sciencesgeneticschromosomes Programme(s) FP3-ENV 1C - Specific research and technological development programme (EEC) in the field of the environment, 1990-1994 Thème(s) 040203 - Environment and human health Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Rijksuniversiteit Leiden Contribution de l’UE Aucune donnée Adresse 72,Wassenaarseweg 2300 RA Leiden Pays-Bas Voir sur la carte Coût total Aucune donnée Participants (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire KAROLINSKA INSTITUTE Suède Contribution de l’UE Aucune donnée Adresse Nobels Vaeg, 13 171 77 STOCKHOLM Voir sur la carte Coût total Aucune donnée Universität Bremen Allemagne Contribution de l’UE Aucune donnée Adresse Bibliothekstraße 1 28334 Bremen Voir sur la carte Coût total Aucune donnée
