Objetivo Characterization of a range of triosephophate isomerases (TIMs) which differ widely in stability. Creation of monomeric mutants of TIM by either site directed mutagenesis or complexation with tight binding cyclopeptides, or via both approaches. Eventually, with the help of model building techniques, the implementation of loop transplantations will be attempted in such a way that single monomers are formed with an altered specificity and/or new catalytic activity. The current objectives involve research on: trypanosomal TIM; human TIM; Escherichia coli TIM; octarellin design; psychrophilic TIM and thermophilic TIM; cyclopeptides; and loop transplantation modelling studies.Major results to date include: TIM variants of trypanosomal TIM and E coli TIM have been made and partly characterized;the sequences of TIM from thermophilic Bacillus Stearothermophilus and psychrotrophic Moraxella spp TA137 have been determined;the structural change from an oxopeptide to the thiopeptide cyclo(Pheomega [CSNH]-Phe-Gly-Pro-Phe-Val-) results in a drastic increase in biological activity;the crystal structure of wild type E coli TIM has been determined at 2.6 angstroms resolution.A network of European research groups has been formed to study and engineer the stability and activity of the dimeric enzyme triosephosphate isomerase (TIM) with the ultimate goal of being able to create new classes of enzymes based on the (beta alpha)8-framework. Within this collaboration psychrotrophic and thermophylic TIMs will be studied, including the crystal structure determinations. This data will help to evaluate important characteristics related to the stability of this enzyme. On the basis of the refined crystal structure of trypanosomal TIM, mutants will be made in which the dimer interface interactions are weakened. The eventual goal is to obtain monomeric TIM (complexed with or without cyclopeptides which mimic the dimer interface loops) with an altered specificity and/or new catalytic activity. Ámbito científico engineering and technologymaterials engineeringcrystalsmedical and health sciencesclinical medicinetransplantationnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP2-BRIDGE - Specific research and technological development programme (EEC) in the field of biotechnology (BRIDGE), 1990-1994 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador EUROPEAN MOLECULAR BIOLOGY LABORATORY Aportación de la UE Sin datos Dirección Meyerhofstrasse 1 HEIDELBERG Alemania Ver en el mapa Coste total Sin datos Participantes (5) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo CHRISTIAN DE DUVE INSTITUTE OF CELLULAR PATHOLOGY Bélgica Aportación de la UE Sin datos Dirección 74,Avenue Hippocrate 74, ICP-TROP 74.39 1200 BRUXELLES Ver en el mapa Coste total Sin datos Rijksuniversiteit Groningen Países Bajos Aportación de la UE Sin datos Dirección 16,Nijenborgh 9747 AG Groningen Ver en el mapa Coste total Sin datos Société Européenne de Biotechnologie SA Bélgica Aportación de la UE Sin datos Dirección 14,Rue Bois Saint-Jean 4102 Seraing Ver en el mapa Coste total Sin datos Technische Universität München Alemania Aportación de la UE Sin datos Dirección Lichtenbergstraße 4 85748 Garching bei München Ver en el mapa Coste total Sin datos Université de Liège Bélgica Aportación de la UE Sin datos Dirección Campus du Sart Tilman 4000 Liège Ver en el mapa Coste total Sin datos