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Zawartość zarchiwizowana w dniu 2022-12-23

Biogenesis and stability of the photosynthetic apparatus in Gymnosperms under normal and stress conditions

Cel

- To examine chlorophyll synthesis in gymnosperms and define the "dark synthesis" pathway for evaluating the relative contribution of this pathway to chlorophyll synthesis in response to temperature variations and to atmospheric pollutants;
- To examine how chlorophyll synthesis is integrated with chlorophyll assimilation into photosynthetic complexes in gymnosperms compared to angiosperms. This will allow to understand how chlorophyll synthesis and the assembly of particular chlorophyll-protein complexes (light-harvesting and reaction centre complexes) are coupled;
- To characterise the photosynthetic physiology of gymnosperms at a molecular and biochemical level and make comparisons to other plants;
- To investigate at a molecular level the effects of environmental stress on photosynthetic apparatus of gymnosperms. This aims at determining the effects of environmental stresses (extreme temperatures and irradiances, sulphur dioxide) on the accumulation of free pigment that may cause photo-oxidative damage and at identifying how these factors interfere with the normal turn-over of photosynthetic components.
- Measurements of chlorphyllase activity in various plant materials including pine.
- Evidence for a role of beta-carotene in photoinhibition and rapid turn-over of the D1 protein of photosystem II reaction centre in Chlamydomonas reinhardtii.
- Analysis of the characteristics of chlorophyll fluorescence lifetime during assembly of photosystems in darkness and further activation by continuous light in cotyledons of Pinus brutia.
- Characterisation of the development of the photosynthetic apparatus in seedlings of Pinus jeffreyi and Pinus brutia by low temperature, steady-sate fluorescence spectroscopy.
- Determination of the influence of the photoactivation of photosystem II on the characteristics of constant and variable fluorescence of chlorophyll at room temperature in cotyledons of Pinus jeffreyi and Pinus brutia.
- Mathematical analysis of spectroscopic characteristics of in vivo protochlorophyll(ide) forms during dark-growth of pine seedlings. Detection of three different forms of photo-inactive and two forms of photo-active protochlorophyll(ide). Evidence of an enhancement by low temperature treatments of the light-dependent pathway of chlorophyll synthesis in Pinus jeffreyi.
- Determination by high performance liquid chromatography of the pigment composition (carotenoids and chlorophyll's) in cotyledons and needles of dark-grown seedlings of Pinus jeffreyi and Pinus sylvestris.

- Pine (seedlings and needles from mature tress) is used as representative gymnosperm. The principal material for comparison (representative angiosperm) is barley. The green algae Chlamydomonas reinhardtii is used for specific purposes;
- Investigations on the mechanism of light-independent protochlorophyllide reduction in gymnosperms;
- Spectroscopic studies on protochlorophyllide forms in pine seedlings and their formation at normal or low temperature;
- Studies on the regulation of photosynthetic chlorophyll-protein complexes assembly in gymnosperms and angiosperms (free pigment accumulation, photochemical activities, polypeptide synthesis);
- Studies on the mechanism of photoinhibition and recovery processes in relation to chlorophyll turn-over;
- Investigations on the relationship between carotenoid synthesis and photosystem II assembly and turn-over;
- Study of pollutant effects (sulphur dioxide) on chlorophyll synthesis and integration into pigment-protein complexes.

Temat(-y)

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Zaproszenie do składania wniosków

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System finansowania

CSC - Cost-sharing contracts

Koordynator

Université de Liège
Wkład UE
Brak danych
Adres
27,Boulevard du Rectorat
4000 Liège
Belgia

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Koszt całkowity
Brak danych

Uczestnicy (1)