Objective The objective of this additional proposal is to characterize a portion of human chromosome 21 (21q22) using technology that is being developed in the project of the coordinator. The additional proposal Will test the reliability and day-to-day usefulness of the newly developed technologies in characterizing human genome. Since We are interested in studying abnormal expression of human cystathionine beta-synthase (CBS) We propose to analyze region 21q22.3 containing this gene. In the study We Will employ the YAC and pl phage libraries to obtain clones positive for CBS. We will further characterize the gene bY restriction mapping' splicing-Alu PCR' splicing-exon PCR' and automated DNA sequencing. Additional studies Will include the search for microsatellites and polymorphic markers and also functional analysis of CBS MRNA isoforms. Individual tasks of the project and data analysis Will be carried out in close collaboration among the proposing laboratory, coordinator and prof.Estivill's laboratory. Programme(s) IC-PECO/COPERNICUS - Scientific and technological cooperation between the European Community and European non-member countries, 1992- Topic(s) 0202 - CEEC participation in BIOMEDICAL AND HEALTH RESEARCH programme (HUMAN GENOME) Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator EUROPEAN MOLECULAR BIOLOGY LABORATORY EU contribution No data Address Meyerhofstrasse 1 HEIDELBERG Germany See on map Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Charles University Czechia EU contribution No data Address 5,U Nemocnice 128 53 Praha 1 See on map Total cost No data
