Objective The main goal of this project is to develop basic means for in-depth understanding of viral diseases affecting commercially exploited molluscs. This will be achieved through the development of functional mollusc specific expression vectors and subsequently by the obtention of mollusc continuous cell lines. We will study Mytilus edulis since it is widely used as an experimental model. The project will comprise six main experiments; some of them can be initiated independently and therefore allow some flexibility in the realisation of this project. The goal is to be reached through a collaborative effort between the participants using complementary approaches. Experiment 1: Cloning of mussei genomic DNA. Isolation and characterization of mussel genes. Experiment 2: Mussel specific expression vectors. Experiment 3: Establishement of primary cell culture system from the mussel Mytilus edulis. Experiment 4: Expression of oncogenes in mussel cells. Experiment 5: Isolation of genetic markers. Experiment 6: Stable cell lines from Mytilus edulis. Fields of science natural sciencesbiological sciencesgeneticsDNA Programme(s) FP3-AIR - Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994 Topic(s) 255 - Improvement and conservation of the genetic basis of aquaculture Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) EU contribution No data Address Université de Montpellier II 2 place Eugène Bataillon 34095 Montpellier France See on map Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSIDADE DO ALGARVE Portugal EU contribution No data Address 322,Campus de Gambelas 8000 FARO See on map Total cost No data
