Objetivo This T project provides a framework that links scientists working on methods for gene identification to scientists dedicated to answering fundamental questions in plant biology using genetics and physiology as their disciplines. This linkage will accelerate the dissemination of techniques for gene search, and their applications to the molecular characterization of genes involved in flowering, seed development and embryogenesis. Some of the major scientific goals met in the Arabidopsis thaliana programme are as follows. Approximately one third of the genome has been mapped, techniques for rapidly screening yeast artificial chromosome (YAC) libraries have been developed, and a database has been established for collating mapping and genetic data. Useful frequencies of homologous recombination in Arabidopsis have been demonstrated using the reconstruction of a hygromycin resistence gene as a selection for these rare events. These constructs are being made available for testing recombination methods and deoxyribonucleic acid (DNA) delivery systems. Important new mutations affecting abscisic acid action have been isolated, and the effect of these on gene expression, lipid and sugar metabolism, and interactions with other mutants are being investigated. An 11 kb piece of DNA has been identified, using chromosome walking, that complement abi3 mutations. With this gene identified, its sequence will help reveal the mode of action of a key growth regulator in plants. T-DNA tagged loci have been identified that affect the tissue specificity of GUS expression. Several of these effect embryo specific patterns of expression. A gene encoding the APETALATA2 product has been tagged using T-DNA and isolated. This gene, which determines floral morphology together with a small group of other homeotic genes, has been further characterised by sequencing. A walk using YACs of about 1 600kb has covered the co locus, which conditions flowering time. Several recombinants have narrowed down the region containing the gene, and complementation experiments are underway to isolate clones containing the gene. Similar walks are progressing towards the fca, fwa and fve loci. In addition, epistatic interactions between the different classes of mutants are being studied. Systems that drive high frequency excision and methods for selecting reintegration events, in transposon tagging have been developed. Large populations of plants are being screened for mutations.This is a coordinated proposal from 44 participants in 9 countries that aims to develop techniques for gene isolation from Arabidopsis and use these methods for isolating genes involved in different aspects of plant biology relevant to agricultural development. There are 7 industrial contractors who will contribute directly to the proposal. Refinement of Arabidopsis as a genetic tool will be accomplished by groups for physical mapping, transposon tagging, gene replacement and resource distribution. Surrounding this are 5 groups who propose to concentrate their efforts on floral initiation, plant growth and differentiation, metabolic control, disease resistance, and seed development and stress responses. The proposed groups are based on areas of common technical or biological expertise and aims, and have been coordinated to provide a balance between technological and biological development that will, if implemented, lead to the rapid development of Arabidopsis as a tool for isolating genes of interest to agriculture. This framework has been designed to accommodate further biological areas of agricultural interest. Existing international collaborations Will be strengthened by this framework and will provide an efficient approach to the physical mapping and cloning of the Arabidopsis genome. At the end of the proposed work there will be sufficient data to initiate a programme of ordered sequencing of the entire genome. Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomes Programa(s) FP2-BRIDGE - Specific research and technological development programme (EEC) in the field of biotechnology (BRIDGE), 1990-1994 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador JOHN INNES CENTRE Aportación de la UE Sin datos Dirección Norwich Research Park, Colney NORWICH Reino Unido Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos Participantes (19) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Centre National de la Recherche Scientifique Francia Aportación de la UE Sin datos Dirección Avenue de Villeneuve 66860 Perpignan Ver en el mapa Coste total Sin datos Centre for Plant Breeding Research Países Bajos Aportación de la UE Sin datos Dirección 6,Keijenbergseweg 6700 AA Wageningen Ver en el mapa Coste total Sin datos Consejo Superior de Investigaciones Cientòficas España Aportación de la UE Sin datos Dirección 18-26,Jordi Girona 08034 Barcelona Ver en el mapa Coste total Sin datos DANISCO A/S Dinamarca Aportación de la UE Sin datos Dirección 1,Langebrogade 1 1001 KOEBENHAVN K/COPENHAEGEN Ver en el mapa Coste total Sin datos INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA España Aportación de la UE Sin datos Dirección KM.7,Carretera de La Coruça KM.7 28040 MADRID Ver en el mapa Coste total Sin datos Institut für Genbiologische Forschung Berlin GmbH Alemania Aportación de la UE Sin datos Dirección Ihnestraße 63 14195 Berlin Ver en el mapa Coste total Sin datos KEYGENE NV Países Bajos Aportación de la UE Sin datos Dirección 90, 90 Agro business Park 6700 AE WAGENINGEN Ver en el mapa Coste total Sin datos LEIDEN UNIVERSITY Países Bajos Aportación de la UE Sin datos Dirección 64,Wassenaarseweg 64 2333 AL LEIDEN Ver en el mapa Coste total Sin datos Max-Planck-Gesellschaft zur Forderungder Wissenschaften e.V. Alemania Aportación de la UE Sin datos Dirección Karl-von-Linné-Weg 10 50829 Köln Ver en el mapa Coste total Sin datos Mogen International NV trading as ZENECA MOGEN Países Bajos Aportación de la UE Sin datos Dirección 97,Einsteinweg 2333 CB Leiden Ver en el mapa Coste total Sin datos Plant Genetics Systems NV Bélgica Aportación de la UE Sin datos Dirección 22,Jozef Plateaustraat 9000 Gent Ver en el mapa Coste total Sin datos SG Seeds BV Países Bajos Aportación de la UE Sin datos Dirección 62,Westeinde 1600 AA Enkhuizen Ver en el mapa Coste total Sin datos SWISS FEDERAL INSTITUTE OF TECHNOLOGY ZUERICH Suiza Aportación de la UE Sin datos Dirección 2,Universitaetstrasse 2 8092 ZURICH Ver en el mapa Coste total Sin datos THE PROVOST, FELLOWS AND SCHOLARS OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN HEREINAFTER TRINITY COLLEGE DUBLIN Irlanda Aportación de la UE Sin datos Dirección Smurfit Institute,Trinity College 2 DUBLIN Ver en el mapa Coste total Sin datos Universiteit Gent Bélgica Aportación de la UE Sin datos Dirección 35,K.L. Ledeganckstraat 35 9000 Gent Ver en el mapa Coste total Sin datos University of Leicester Reino Unido Aportación de la UE Sin datos Dirección University Road LE1 7RH Leicester Ver en el mapa Coste total Sin datos University of Nottingham Reino Unido Aportación de la UE Sin datos Dirección University Park NG7 2RD Nottingham Ver en el mapa Coste total Sin datos WAGENINGEN UNIVERSITY Países Bajos Aportación de la UE Sin datos Dirección 4,Arboretumlaan 4 6703 BD WAGENINGEN Ver en el mapa Coste total Sin datos Zeneca Group plc Reino Unido Aportación de la UE Sin datos Dirección Fernhurst GU27 3JE Haslemere Ver en el mapa Coste total Sin datos