New electrophoretic strategies for faster sequencing,mapping and DNA assay

Projektinformationen

ID Finanzhilfevereinbarung: CIPD940409

Startdatum 1 Juni 1995

Enddatum 30 November 1996

Finanziert unter

• Scientific and technological cooperation between the European Community and European non-member countries, 1992-

Gesamtkosten € 0,00

EU-Beitrag Keine Daten

Koordiniert durch

Centre National de la Recherche Scientifique (CNRS)
France

Ziel

The project proposes theoretical studies of electrophoretic transport phenomena in free fluid and viscous sieving media applied to DNA separation within the frame of the project New Electrophoretic Strategies for Faster Sequencing, Mapping and DNA Assay (NEST) directed by J.L.Viovy. The main aim of the present project is the improverment of existing and the development of new mathematical models describing electrophoretic migration and its computer simulation based on these models. The accent will be on quantitative rather than qualitative agreement with experimental data.
The efforts within this project will concentrate on the following tasks: --- The development of an advanced theoretical model for electrophoretic transport based on continuous media mechanics approach in order to account more accurately for the properties of separation media (high viscosity sieving and possible non-newtonian behavior). Different separation modes will be tried (zone electrophoresis the sample stacking by isotachophoresis) Results of computer modelling will be compared with experimental data. Of primary interest are the effects of sample overloading buffer discontinuities and sieving properties. These circumstances may produce the greatest effect On separation efficiency.
--- Electrokinetic loading as a unique mode for sample injection in viscous solutions. It will be simulated accounting for the presence of salts in the sample and for their effects the load-ability on the DNA fragments.
--Quantitation procedure. Precise assessment of sample characteristics based on electropherogram data will be developed. It will be used for quantitative studies of automated injection systems in order to improve its characteristics. The theoretical studies based on computer simulations have a good chance for success since they use already existing highly efficient numerical techniques. The computer simulation will be done with continuous comparison of experimental studies thus providing the feedback for further improvements of the theoretical model.

Programm/Programme

• IC-PECO/COPERNICUS - Scientific and technological cooperation between the European Community and European non-member countries, 1992-

Thema/Themen

• 0202 - CEEC participation in BIOMEDICAL AND HEALTH RESEARCH programme (HUMAN GENOME)

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

CSC - Cost-sharing contracts

Koordinator

Centre National de la Recherche Scientifique (CNRS)

• EU-Beitrag: Keine Daten
• Adresse: 10 rue Vauqelin ; 75231 Paris ; Frankreich
• Gesamtkosten: Keine Daten

Beteiligte (1)

Keldysh Institute of Applied Mathematics - Russian Academy of Sciences

Russland

• EU-Beitrag: Keine Daten
• Adresse: 4,Myusskaia Sq. ; 125047 Moskva
• Gesamtkosten: Keine Daten