Objectif The objective of this programme is to facilitate the use of homologous recombination in the rat. The long-term aim is achieve a better understanding of the role of specific genes in normal and abnormal blood pressure regulation.The rat is the species of choice for many areas of biomedical research, including hypertension and neurobiology, however existing techniques of germline modification in this species are limited. Although it is technically possible to produce transgenic rats by DNA microinjection, the ability to introduce precise genetic alterations is severely limited by the lack of suitable embryonic stem cells. To date all attempts to isolate such cell lines have not resulted in successful germline transmission. Further efforts and new approaches are therefore required to obtain suitable cell lines. Our aim is to develop a generic method of ES cell derivation through a selection-based approach in order to extend stem cell technology into this species. If the selection methodology is successful this would eventually permit germline manipulation of existing phenotypically selected models of hypertension such as the Spontaneously Hypertensive Rat (SHR), and would enable the future developments to other rat strains of interest to biomedical researchers studying of human disease. Champ scientifique natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesgeneticsDNAmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicinecardiologycardiovascular diseases Programme(s) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Thème(s) 4.3.3 - Genetic bases of cardiovascular diseases Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur University of Edinburgh Contribution de l’UE Aucune donnée Adresse West Main Road EH9 3JQ EDINBURGH Royaume-Uni Voir sur la carte Coût total Aucune donnée Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Université Claude Bernard Lyon 1 France Contribution de l’UE Aucune donnée Adresse AVENUE ROCKEFELLAR 69373 LYON Voir sur la carte Coût total Aucune donnée
