Obiettivo The objective of this programme is to facilitate the use of homologous recombination in the rat. The long-term aim is achieve a better understanding of the role of specific genes in normal and abnormal blood pressure regulation.The rat is the species of choice for many areas of biomedical research, including hypertension and neurobiology, however existing techniques of germline modification in this species are limited. Although it is technically possible to produce transgenic rats by DNA microinjection, the ability to introduce precise genetic alterations is severely limited by the lack of suitable embryonic stem cells. To date all attempts to isolate such cell lines have not resulted in successful germline transmission. Further efforts and new approaches are therefore required to obtain suitable cell lines. Our aim is to develop a generic method of ES cell derivation through a selection-based approach in order to extend stem cell technology into this species. If the selection methodology is successful this would eventually permit germline manipulation of existing phenotypically selected models of hypertension such as the Spontaneously Hypertensive Rat (SHR), and would enable the future developments to other rat strains of interest to biomedical researchers studying of human disease. Campo scientifico natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesgeneticsDNAmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicinecardiologycardiovascular diseases Programma(i) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Argomento(i) 4.3.3 - Genetic bases of cardiovascular diseases Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore University of Edinburgh Contributo UE Nessun dato Indirizzo West Main Road EH9 3JQ EDINBURGH Regno Unito Mostra sulla mappa Costo totale Nessun dato Partecipanti (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Université Claude Bernard Lyon 1 Francia Contributo UE Nessun dato Indirizzo AVENUE ROCKEFELLAR 69373 LYON Mostra sulla mappa Costo totale Nessun dato