Objective In the first place organic synthetic work will be carried out on one or two purine nucleoside analogues to incorporate them into oligonucleotides. The particular structures will be chosen to give them the character of hydrogen-bond ambiguity (or degenerate). The nucleotides will be studied carefully by spectroscopic and other methods to give a detailed picture of their tautomeric states. This will be correlated with the stabilities (thermal dissociation temperatures) of oligonucleotide duplexes containing them and a full thermodynamic study will be made. Such oligonucleotides will be studied as hybridisation primers for polymerase chain reaction (PCR) amplification of genomic DNA. Earlier work has shown the feasibility of using this in gene probing. Its extension to a generally applicable methodology for use by molecular biologists is the expected outcome. The application of such oligomers, in which the degenerate residues act as neutral bases in 2D-assays for DNA sequencing by hybridisation is an important objective. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 42 - Physical Chemical Biology Call for proposal Data not available Funding Scheme Data not available Coordinator MRC Laboratory of Molecular Biology EU contribution No data Address Hills Road CB2 2QH Cambridge United Kingdom See on map Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all Medical Research Council (MRC) United Kingdom EU contribution No data Address 20 Park Crescent W1N 4AL London See on map Total cost No data Russian Academy of Sciences Russia EU contribution No data Address 117984 Moscow See on map Total cost No data
