Objective The first part of this project is directed to the structural study of a new family of presynaptic proteins cloned by CNRS. This new family of presynaptic proteins forms a glutamate neuroreceptor when expressed in vitro. Methods will include a construction of neuroreceptor when expressed in vitro; a construction of recombinant plasmids based on yeast expression vectors and purification of the proteins. Further crystallographic analysis of the protein structure will be performed at the Medical Research Council Centre in the United Kingdom. The second part of the project is in line with previous studies performed at CNRS,France, and concerns the study of the promoter region of the gene coding for a neurotransmitter biosynthetic enzyme tyrosine hydroxylase (TH). The project will be directed to the cloning of neuronal transcription factors, which recognise specific sites of the TH promoter, by using a collection of yeast mutants and expression vectors. Secondly, the techniques developed at CNRS and at St. Petersburg University, will allow identification and isolation of different members of the multimetric protein complexes involved in TH promoter activity. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 44 - Biotechnology Call for proposal Data not available Funding Scheme Data not available Coordinator Centre National de la Recherche Scientifique EU contribution No data Address Avenue de la Terrasse 1 91198 Gif-sur-Yvette France See on map Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all MRC Laboratory of Molecular Biology United Kingdom EU contribution No data Address Hills Road CB2 2QH Cambridge See on map Total cost No data St. Petersburg State University Russia EU contribution No data Address 198904 St. Petersburg See on map Total cost No data