Cel An understanding of cellular processes at the molecular level is the basic knowledge that underpins diagnosis and therapy in human medicine. Gene transcription and its control is a key process, not least because cancer results from aberrant gene expression. The protein HMG1 plays a general, but as yet poorly understood, role in gene transcription and this research project will further the understanding of its function. Since it bends through a large angle on binding, it exhibits very special properties. HMG1 contains two HMG boxes, a new DNA binding motif found in other transcriptional activators, and therefore all the findings should have a broader application. Mapping the location of HMG1/2 in the genome will be a major task. Therefore monoclonal and polyclonal HMG 1 antibodies will be produced and checked using the Western blot method. DNA / HMG1 complexes will be transfected into mammalian cells and the efficiency of the transfection will be studied. The structure of the DNA / HMG1 complex will be investigated usibng optical (CD) and biochemical (EMSA) methods. The complex will also be studied by measurement of the fluorescent intensity and of the fluorescent resonant energy transfer. Program(-y) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Temat(-y) 44 - Biotechnology Zaproszenie do składania wniosków Data not available System finansowania Data not available Koordynator University of Portsmouth Wkład UE Brak danych Adres White Swan Road PO1 2DT Portsmouth Zjednoczone Królestwo Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (4) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Academy of Sciences of Georgia Gruzja Wkład UE Brak danych Adres 380077 Tbilisi Zobacz na mapie Koszt całkowity Brak danych Max-Delbrück-Centre for Molecular Medicine Niemcy Wkład UE Brak danych Adres 13125 Berlin Zobacz na mapie Koszt całkowity Brak danych National Kiev Taras Shevchenko University Ukraina Wkład UE Brak danych Adres 252017 Kiev Zobacz na mapie Koszt całkowity Brak danych Russian Academy of Sciences Rosja Wkład UE Brak danych Adres 194064 St. Petersburg Zobacz na mapie Koszt całkowity Brak danych