Objectif The project is focused on in vivo translational control and its role in physiological processes in eukaryotic cells. Primary studies will be concentrated on the SUP35 omnipotent suppressor of the yeast Saccharomyces cerevisiae. Former experiments have revealed that this gene is one of the key genetic components controlling translational fidelity in yeast. Recently obtained data also show that the SUP35 gene product is probably a yeast analogue of prion proteins of higher eukaryotes. This enables a convenient model system to be developed for studying mechanisms of protein-based heredity. Finally, the SUP35 gene is of general interest since its homologues were recently found in several higher eukaryotic organisms including man. The basis of this project is to analyze the regulation of expression of the SUP35 gene and to identify the possible function(s) of the corresponding gene product. Special attention will be given to studying the relation of the SUP35 gene product to the non-Mendelian determinant [psi], the probable analogue of prion proteins of higher eukaryotes. All these problems will be investigated through several approaches involving the specialized abilities of the different participating laboratories. A homologous cell-free translation system that is dependent on the SUP35p protein will be generated in order to estimate its role in protein synthesis. On the other hand, the identification of genes which interact with SUP35 will be undertaken; this can be done either by searching for genes, the expression of which is under the control of the SUP35 gene product, or by identifying physically interacting components. Several efficient genetic and biochemical methods are already operational. Finally, study of the expression of SUP35 under several conditions, especially stress, will be analyzed and the components responsible for this regulation identified.These various approaches may help in defining the inter-relationships of translation with other cellular processes in yeast. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Thème(s) 44 - Biotechnology Appel à propositions Data not available Régime de financement Data not available Coordinateur Vrije Universiteit Amsterdam Contribution de l’UE Aucune donnée Adresse De Boelelaan 1083 1081 HV Amsterdam Pays-Bas Voir sur la carte Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Institute of Experimental Cardiology Russie Contribution de l’UE Aucune donnée Adresse 121552 Moscow Voir sur la carte Coût total Aucune donnée University of Kent at Canterbury Royaume-Uni Contribution de l’UE Aucune donnée Adresse CT2 7NJ Canterbury Voir sur la carte Coût total Aucune donnée Université de Paris-Sud - Paris XI France Contribution de l’UE Aucune donnée Adresse 91405 Orsay Voir sur la carte Coût total Aucune donnée
