Obiettivo The aim of this project is the design of interactive antisense oligonucleotides for therapeutic use at physiological temperatures. This requires that the specificity of hybridisation be maintained and the thermal behaviour, i.e. the discriminatory temperature range near melting, adapted to physiological conditions. As a route to achieving this goal, chimeric oligonucleotide-oligoethylene glycol oligoether-phosphates are prepared. In these chimeric compounds, the oligonucleotide segments will be short enough to ensure melting temperatures near physiological, whereas the total length of base pairing will be maintained around 17-20 bases to ensure maximum specificity. The oligoethylene glycol moieties will not only serve as spacers between the oligonucleotide segments, but may also enhance the cellular uptake of these macromolecules. The conjugation to organic substituents will be studied as follows: modulate oligonucleotide hybridisation (e.g. phenazinium residues); account for improved uptake, and produce cytotoxic effects in target cells by, non-triggered interaction with nucleic acids through alkylating residues or by triggered cleavage of nucleic acids by hydrophilic porphine moieties. Programma(i) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Argomento(i) 36 - Biochemistry and New Pharmaceutical Compounds Invito a presentare proposte Data not available Meccanismo di finanziamento Data not available Coordinatore Universität Ulm Contributo UE Nessun dato Indirizzo Albert-Einstein-Allee 11 89069 Ulm Germania Mostra sulla mappa Costo totale Nessun dato Partecipanti (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto Novosibirsk Institute of Bioorganic Chemistry Russia Contributo UE Nessun dato Indirizzo 630090 Novosibirsk Mostra sulla mappa Costo totale Nessun dato UNIVERSITA DEGLI STUDI DI PADOVA Italia Contributo UE Nessun dato Indirizzo Via F. Marzolo 1 35131 PADOVA Mostra sulla mappa Costo totale Nessun dato