Obiettivo In contrast to the wealth of information gathered in neuropsychology since the advent of imaging technologies, the cellular physiology of the neocortex is yet underdeveloped. The aim of this work is specifically to understand what determines the functions and the characteristics of excitatory synapses between pyramidal neurons and the different types of interneurons of the neocortex. This will be achieved by combining electrophysiological recordings of pairs of neurons with calcium imaging at the single synapse level using multiphoton microscopy, single RT-multiplexPCR and anatomical reconstruction of the connected neurons. The calcium imaging will detect transients in both pre- and postsynaptic elements and involve the development of new multiphoton laser scanning microscope hard- and software. The single cell RT-multiplexPCR will be expanded using DNA microchip technology to detect the expression of 100 genes relevant to cell-type identification and synaptic transmission. Campo scientifico natural sciencescomputer and information sciencessoftwarenatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesinorganic chemistryalkaline earth metalsnatural sciencesphysical sciencesopticsmicroscopynatural sciencesphysical sciencesopticslaser physics Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-9. - Neurosciences Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contributo UE Nessun dato Indirizzo Rue Michel Ange 3 75794 PARIS Francia Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto LEICA MICROSYSTEMS HEIDELBERG Germania Contributo UE Nessun dato Indirizzo Im Neuenheimer Feld 518 69120 HEIDELBERG Mostra sulla mappa Costo totale Nessun dato MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Germania Contributo UE Nessun dato Indirizzo Jahnstraße 29 69120 Heidelberg Mostra sulla mappa Costo totale Nessun dato
