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Validation and standardisation of diagnostic polymerase chain reaction for detection of food-borne pathogens (FOOD-PCR)

Project information

Grant agreement ID: QLK1-CT-1999-00226

  • Start date

    1 March 2000

  • End date

    30 June 2003

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 2 326 333

  • EU contribution

    € 1 607 220

Coordinated by:

DANISH INSTITUTE FOR FOOD AND VETERINARY RESEARCH

Denmark

Objective

TECHNOLOGICAL PROBLEM

Recognising that sensitive and more cost-effective methods are needed for detecting food-borne pathogens, we are launching a project seeking to validate and standardise use of the polymerase chain reaction (PCR) for this purpose. Although a powerful research tool, the application of PCR for detecting food-borne pathogens is hampered from lack of validation, standard protocols, reagents, and equipment. Additional specific project objectives include validating a simple method for purifying DNA from bacterial cultures, establishing a central collection of certified DNA sample materials, establishing a databank containing key food-pathogen DNA sequence, listing strains for specificity testing, developing standardised reagents, and validating pre-PCR sample treatment methods.

EXPECTED RESULTS

To facilitate its routine use for diagnostic purposes, participants in the new project are being asked to undertake a series of specific projects, including to construct a DNA sample library and primer databank, validate widely used thermocyclers and other automated equipment, and to develop uniform guidelines describing how tests should be conducted. Because much of the challenge in applying PCR to food-borne pathogens is technical, there is also a need to develop standardised training manuals and procedures for those who will conduct such tests.

APPLICATIONS

The program is planned in 2 phases over a 3-year period and will include 6 work packages and 20 tasks. During phase 1, researchers working in 12 separate laboratories will develop data about the basic methods and requisite reagents. During phase 2, they will focus on validation trials, automation issues, and transferring the validated technology to end-users.

As part of this project, 35 participants are being asked to focus on 5 major pathogens--Salmonella, Campylobactor, enterohemorrhagic E. coli (EHEC), L. monocytogenes and Y. enterocolitica, and on three sample types: poultry carcass-rinse, pig carcass swab, and milk. An expert laboratory is assigned to each pathogen, with the task to prepare defined DNA material, compare the methods and devise and optimise a PCR detection protocol.

Coordinator

DANISH INSTITUTE FOR FOOD AND VETERINARY RESEARCH

Address

Bulowsvej 27
1790 Koebenhavn V

Denmark

Participants (32)

AGENCE FRANCAISE DE SECURITE SANITAIRE DES ALIMENTS

France

AGRICULTURAL RESEARCH CENTRE-GENT

Belgium

BIOTECON DIAGNOSTICS GMBH

Germany

COMMISSION OF THE EUROPEAN COMMUNITIES

Belgium

CONGEN BIOTECHNOLOGIE GMBH

Germany

COOP ITALIA

Italy

DANISH MEAT RESEARCH INSTITUTE

Denmark

DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS

United Kingdom

DIFFCHAMB AB

Sweden

ESTONIAN AGROBIOCENTRE

Estonia

FEDERAL INSTITUTE FOR HEALTH PROTECTION OF CONSUMERS AND VETERINARY MEDICINE

Germany

FOOD RESEARCH INSTITUTE

Slovakia

Head of the Unit of Foodborne Zoonoses and Veterinary Epidemiology

Italy

INSPECTORATE FOR HEALTH PROTECTION, COMMODITIES AND VETERINARY PUBLIC HEALTH

Netherlands

INSTITUTE OF ANIMAL PHYSIOLOGY - SLOVAK ACADEMY OF SCIENCES

Slovakia

INSTITUTE OF CHEMICAL TECHNOLOGY PRAGUE

Czechia

INSTITUTO DE RECERCA I TECNOLOGIA AGROALIMENTARIES

Spain

JUSTUS-LIEBIG-UNIVERSITY OF GIESSEN

Germany

LUND UNIVERSITY

Sweden

MATFORSK - NORWEGIAN FOOD RESEARCH INSTITUTE

Norway

MEDICANALYSIS LTD

Greece

NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS

Greece

NATIONAL INSTITUTE OF PUBLIC HEALTH

Czechia

NATIONAL LABORATORY FOR ENGINEERING AND INDUSTRIAL TECHNOLOGY

Portugal

NATIONAL VETERINARY AND FOOD RESEARCH INSTITUTE

Finland

NATIONAL VETERINARY RESEARCH INSTITUTE

Poland

NESTEC S.A.

Switzerland

QUINTESSENCE RESEARCH AB

Sweden

TEAGASC - AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY

Ireland

UNIVERSIDAD COMPLUTENSE DE MADRID

Spain

UNIVERSITE DE LIEGE*ULG

Belgium

UNIVERSITY OF VETERINARY MEDICINE VIENNA

Austria

Project information

Grant agreement ID: QLK1-CT-1999-00226

  • Start date

    1 March 2000

  • End date

    30 June 2003

Funded under:

FP5-LIFE QUALITY

  • Overall budget:

    € 2 326 333

  • EU contribution

    € 1 607 220

Coordinated by:

DANISH INSTITUTE FOR FOOD AND VETERINARY RESEARCH

Denmark